PMID- 24577229
OWN - NLM
STAT- MEDLINE
DCOM- 20141201
LR  - 20220408
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 37
IP  - 2
DP  - 2014 Mar
TI  - Maternal exposure to titanium dioxide nanoparticles during pregnancy; impaired 
      memory and decreased hippocampal cell proliferation in rat offspring.
PG  - 617-25
LID - S1382-6689(14)00015-5 [pii]
LID - 10.1016/j.etap.2014.01.014 [doi]
AB  - Titanium dioxide nanoparticles (TiO2-NPs) are massively produced in the 
      environment, and because of their wide usage, they are a potential risk of damage 
      to human health. TiO2-NPs are often used as additives for paints, papers, and 
      foods. The central nervous system (CNS), including hippocampal regions, is 
      potentially susceptible targets for TiO2-NPs. This study aimed to determine the 
      effects of exposure to TiO2-NPs during pregnancy on hippocampal cell 
      proliferation and the learning and memory of offspring. Pregnant Wistar rats 
      received intragastric TiO2-NPs (100 mg/kg body weight) daily from gestational day 
      (GD) 2 to (GD) 21. Animals in the control group received the same volume of 
      distilled water via gavage. After delivery, the one-day-old neonates were deeply 
      anesthetized and weighed. They were then killed and the brains of each group were 
      collected. Sections of the brains from the rat offspring were stained using Ki-67 
      immunolabeling and the immunohistochemistry technique. Some of the male offspring 
      (n=12 for each group) were weaned at postnatal day (PND21), and housed until 
      adulthood (PND60). Then the learning and memory in animals of each group were 
      evaluated using passive avoidance and Morris water maze tests. The immunolabeling 
      of Ki-67 protein as a proliferating cell marker showed that TiO2-NPs 
      significantly reduced cell proliferation in the hippocampus of the offspring 
      (P<0.05). Moreover, both the Morris water maze test and the passive avoidance 
      test showed that exposure to TiO2-NPs significantly impaired learning and memory 
      in offspring (P<0.05). These results may provide basic experimental evidence for 
      a better understanding of the neurotoxic effects of TiO2-NPs on neonatal and 
      adult brains.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Mohammadipour, Abbas
AU  - Mohammadipour A
AD  - Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
FAU - Fazel, Alireza
AU  - Fazel A
AD  - Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
FAU - Haghir, Hossein
AU  - Haghir H
AD  - Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran; Medical Genetic Research Center (MGRC), School 
      of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
FAU - Motejaded, Fatemeh
AU  - Motejaded F
AD  - Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
FAU - Rafatpanah, Houshang
AU  - Rafatpanah H
AD  - Immunology Research Center, Buali Institute, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Zabihi, Hoda
AU  - Zabihi H
AD  - Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
FAU - Hosseini, Mahmoud
AU  - Hosseini M
AD  - Neurocognitive Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
FAU - Bideskan, Alireza Ebrahimzadeh
AU  - Bideskan AE
AD  - Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of 
      Medical Sciences, Mashhad, Iran. Electronic address: EbrahimzadehBA@mums.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140130
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 15FIX9V2JP (titanium dioxide)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Animals
MH  - Avoidance Learning/drug effects
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - Hippocampus/cytology/*drug effects/metabolism
MH  - Maternal-Fetal Exchange
MH  - Maze Learning/drug effects
MH  - Memory/*drug effects
MH  - Metal Nanoparticles/*toxicity
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats, Wistar
MH  - Titanium/pharmacokinetics/*toxicity
OTO - NOTNLM
OT  - Hippocampal cell proliferation
OT  - Learning and memory
OT  - Maternal exposure
OT  - Neurotoxicity
OT  - Titanium dioxide nanoparticles
EDAT- 2014/03/01 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/01 06:00
PHST- 2013/07/19 00:00 [received]
PHST- 2014/01/16 00:00 [revised]
PHST- 2014/01/20 00:00 [accepted]
PHST- 2014/03/01 06:00 [entrez]
PHST- 2014/03/01 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S1382-6689(14)00015-5 [pii]
AID - 10.1016/j.etap.2014.01.014 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2014 Mar;37(2):617-25. doi: 
      10.1016/j.etap.2014.01.014. Epub 2014 Jan 30.