PMID- 2457772
OWN - NLM
STAT- MEDLINE
DCOM- 19880929
LR  - 20191029
IS  - 0160-5402 (Print)
IS  - 0160-5402 (Linking)
VI  - 20
IP  - 1
DP  - 1988 Aug
TI  - Relationships of secretagogue-induced cAMP accumulation and acid secretion in 
      elutriated rat gastric parietal cells.
PG  - 57-78
AB  - Centrifugal elutriation was adapted and analyzed as a method to separate rat 
      gastric parietal cells from other fundic mucosal cells. Elutriated parietal cell 
      fractions provided sufficient purity by morphological criteria, and fluorescence 
      activated cell sorting analyses, yield, reproducibility and maintenance of 
      functional responses. These characteristics allowed the study and comparison of 
      the kinetics of histamine, isoproterenol, and forskolin-induced cyclic AMP and 
      14C-amino-pyrine accumulations in the presence of 1-methyl-3-isobutyl xanthine 
      (IBMX) in parietal-cell-rich and parietal-cell-poor fractions. All three acid 
      secretagogues studied produced the same maximal rate of acid secretion as judged 
      by 14C-aminopyrine accumulation. Each secretagogue action peaked at different 
      times and had different accumulation kinetics. For each agonist, cyclic AMP 
      accumulation preceded secretion. However, the rate, extent, and temporal changes 
      of cyclic AMP accumulation were independent of aminopyrine accumulation. HPLC 
      separation and drug inhibition studies of cyclic nucleotide phosphodiesterase 
      activities indicated the presence of multiple, high affinity (Type IV), but not 
      lower affinity (Types I and II), enzyme forms in gastric mucosal cells. IBMX did 
      not distinguish between the two forms, but SQ 65442 and RO 20-1724 were selective 
      inhibitors. Inhibition constants of IBMX for phosphodiesterase hydrolysis agreed 
      closely with its EC50 for histamine-stimulated acid secretion (2-8 microM). 
      Elutriated parietal cells maintained their responses to selective receptor 
      antagonists in the micromolar concentration range.
FAU - Black, E W
AU  - Black EW
AD  - Department of Pharmacology, University of South Alabama, College of Medicine, 
      Mobile 36688.
FAU - Strada, S J
AU  - Strada SJ
FAU - Thompson, W J
AU  - Thompson WJ
LA  - eng
GR  - GM 33538/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pharmacol Methods
JT  - Journal of pharmacological methods
JID - 7806596
RN  - 01704YP3MO (Aminopyrine)
RN  - 1F7A44V6OU (Colforsin)
RN  - 820484N8I3 (Histamine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases)
RN  - L628TT009W (Isoproterenol)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - 3',5'-Cyclic-AMP Phosphodiesterases/metabolism
MH  - Aminopyrine/metabolism
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*metabolism
MH  - Fluorescence
MH  - Gastric Acid/*metabolism
MH  - Gastric Mucosa/cytology/*metabolism/ultrastructure
MH  - Histamine/pharmacology
MH  - In Vitro Techniques
MH  - Isoproterenol/pharmacology
MH  - Male
MH  - Microscopy, Electron
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
AID - 0160-5402(88)90016-2 [pii]
AID - 10.1016/0160-5402(88)90016-2 [doi]
PST - ppublish
SO  - J Pharmacol Methods. 1988 Aug;20(1):57-78. doi: 10.1016/0160-5402(88)90016-2.