PMID- 24578229
OWN - NLM
STAT- MEDLINE
DCOM- 20150416
LR  - 20191210
IS  - 1097-0223 (Electronic)
IS  - 0197-3851 (Linking)
VI  - 34
IP  - 6
DP  - 2014 Jun
TI  - Validation of automatic scanning of microscope slides in recovering rare cellular 
      events: application for detection of fetal cells in maternal blood.
PG  - 538-46
LID - 10.1002/pd.4345 [doi]
AB  - OBJECTIVE: Detection of rare fetal cells (FCs) in the maternal circulation could 
      be used for non-invasive prenatal diagnosis. Considering that FCs in maternal 
      blood are present in extremely low frequency, manual scanning is cumbersome, 
      time-consuming, and unsuitable for clinical applications. As an alternative, we 
      optimized a custom-made classifier for automatic detection of FCs. METHODS: Using 
      MetaSystems' automated platform, we developed a robust detection algorithm and 
      validated its efficiency on retrieval of rare XY cells in a pure population of XX 
      cells. Slides were scanned for presence of predefined XY cells after fluorescence 
      in situ hybridization (FISH) and primed in situ labeling (PRINS). Retrieval of 
      FCs was also performed on samples from maternal blood. RESULTS: The efficiency of 
      detection of rare XY cells was 88% using FISH (117/133) in comparison with 78% 
      (53/68) with PRINS. FC frequencies per 1 mL of maternal blood ranged from 3 to 6 
      FCs in normal pregnancies versus 13 to 21 FCs in Down syndrome pregnancies. 
      CONCLUSION: Automatic scanning was more efficient and consistent than manual 
      scanning for detection of rare FCs and required considerably less operator time. 
      Automatic scanning using FISH is more sensitive than that using PRINS. The study 
      validates automatic scanning retrieval of FCs from maternal blood.
CI  - (c) 2014 John Wiley & Sons, Ltd.
FAU - Emad, Ahmed
AU  - Emad A
AD  - Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health 
      Sciences, Universite de Sherbrooke, Sherbrooke, QC, Canada.
FAU - Bouchard, Eric F
AU  - Bouchard EF
FAU - Lamoureux, Josee
AU  - Lamoureux J
FAU - Ouellet, Annie
AU  - Ouellet A
FAU - Dutta, Aparajita
AU  - Dutta A
FAU - Klingbeil, Uli
AU  - Klingbeil U
FAU - Drouin, Regen
AU  - Drouin R
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20140321
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Blood Cells/*cytology/pathology
MH  - Electronic Data Processing/methods
MH  - Female
MH  - Fetus/*cytology
MH  - Hematologic Tests/methods
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - *Primed In Situ Labeling
MH  - Sensitivity and Specificity
EDAT- 2014/03/01 06:00
MHDA- 2015/04/17 06:00
CRDT- 2014/03/01 06:00
PHST- 2013/12/07 00:00 [received]
PHST- 2014/02/23 00:00 [revised]
PHST- 2014/02/23 00:00 [accepted]
PHST- 2014/03/01 06:00 [entrez]
PHST- 2014/03/01 06:00 [pubmed]
PHST- 2015/04/17 06:00 [medline]
AID - 10.1002/pd.4345 [doi]
PST - ppublish
SO  - Prenat Diagn. 2014 Jun;34(6):538-46. doi: 10.1002/pd.4345. Epub 2014 Mar 21.