PMID- 24579479
OWN - NLM
STAT- MEDLINE
DCOM- 20140401
LR  - 20191210
IS  - 1003-9279 (Print)
IS  - 1003-9279 (Linking)
VI  - 27
IP  - 4
DP  - 2013 Aug
TI  - [Establishment of a detection method for hepatitis B virus large surface protein 
      (HBV-lP)].
PG  - 292-4
AB  - OBJECTIVE: To establish enzyme-linked immunosorbent assay (ELISA) for detection 
      of hepatitis B virus large surface protein(HBV-LP) in serum. METHODS: A sandwich 
      reaction was preformed with horseradish peroxidase labeled monoclonal antibody of 
      HBV-LP as the catalytic enzyme. Several reactions liquid's concentration and 
      reaction conditions were optimized. The method was evaluated in all aspects such 
      as sensitivity, specificity, stability and so on. RESULTS: The detection limit 
      was 5 ng/ml. Interassay and intra-assay RSD were both less than 10%. After stored 
      at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation 
      coefficient higher than 0.98 and RSD lower than 10%. CONCLUSION: Established 
      ELISA for determination of serum HBV-LP has high sensitivity and repeatability. 
      Enzyme-linked immunosorbent assay;
FAU - Zhao, Jing
AU  - Zhao J
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Ma, Hong-Bin
AU  - Ma HB
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Hou, Jun
AU  - Hou J
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Guo, Jing-Xia
AU  - Guo JX
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Xu, Jun
AU  - Xu J
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Song, Yong-Ji
AU  - Song YJ
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Chen, Lin
AU  - Chen L
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Liu, Ai-Xia
AU  - Liu AX
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Liu, Jia
AU  - Liu J
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Wei, Hong-Shan
AU  - Wei HS
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
FAU - Li, Bo-An
AU  - Li BA
AD  - Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, 
      China.
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
JT  - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang 
      bingduxue zazhi = Chinese journal of experimental and clinical virology
JID - 9602873
RN  - 0 (Hepatitis B Surface Antigens)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Hepatitis B/blood/*diagnosis/virology
MH  - Hepatitis B Surface Antigens/*blood
MH  - Hepatitis B virus/*isolation & purification/metabolism
MH  - Humans
EDAT- 2014/03/04 06:00
MHDA- 2014/04/02 06:00
CRDT- 2014/03/04 06:00
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/04/02 06:00 [medline]
PST - ppublish
SO  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Aug;27(4):292-4.