PMID- 24580770
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140306
LR  - 20211021
IS  - 1476-9255 (Print)
IS  - 1476-9255 (Electronic)
IS  - 1476-9255 (Linking)
VI  - 11
IP  - 1
DP  - 2014 Mar 2
TI  - Anti-inflammatory effects of resolvin-D1 on human corneal epithelial cells: in 
      vitro study.
PG  - 6
LID - 10.1186/1476-9255-11-6 [doi]
AB  - BACKGROUND: This study evaluated the anti-inflammatory effects of Resolvin-D1 
      (RV-D1) and its mechanism of action in human corneal epithelial (HCE) cells. 
      METHODS: HCE cells were incubated with different concentrations of RV-D1 for 
      different time periods. Oleic acid (OA) and Dexamethasone (DM) served as negative 
      and positive controls, respectively. Cells were stimulated with 
      polyriboinosinic:polyribocytidylic acids (poly I:C). The protein contents and 
      mRNA expression levels of Tumor necrosis factor-alpha (TNF-alpha), Interleukin (IL)-6, 
      IL-1beta and IL-8 were evaluated with multiplex fluorescent bead immunoassay (FBI) 
      and real time-PCR, respectively. In addition, the expression of inhibitory 
      factor-kappaBalpha (I-kappaBalpha) was evaluated with real time-PCR. RESULTS: The protein level 
      of pro-inflammatory cytokines TNF-alpha, IL-6, IL-1beta and IL-8 significantly increased 
      after stimulation with Poly I:C. RV-D1 treatment at concentration of 1 muM 
      decreased the protein level of TNF-alpha to 20.76 +/- 9.3% (P < 0.05), IL-6 to 
      43.54 +/- 14.16% (P < 0.001), IL-1beta to 46.73 +/- 15.93% (P > 0.05) and IL-8 to 
      51.15 +/- 13.01% (P < 0.05) compared with cells stimulated with poly I:C alone. 
      Similarly, the mRNA levels of TNF-alpha, IL-6, IL-1beta and IL-8 were significantly 
      reduced after treatment with RV-D1. A highly significant dose response curve was 
      demonstrated for RV-D1 treated HCE cells for TNF-alpha and IL-1beta.DM treatment 
      decreased the protein content for all of the pro-inflammatory cytokines, similar 
      results were demonstrated at the mRNA level. The anti-inflammatory effects of 
      RV-D1 were similar to those of DM for TNF-alpha, IL-6 and IL-8. CONCLUSIONS: RV-D1 
      may serve as a potent anti-inflammatory agent in ocular surface inflammation, as 
      evaluated in cultured HCE cells. The anti-inflammatory effects of RV-D1 were 
      comparable to those of DM, and were mediated through nuclear factor kappa B 
      (NF-kappaB) signal transduction.
FAU - Erdinest, Nir
AU  - Erdinest N
FAU - Ovadia, Haim
AU  - Ovadia H
FAU - Kormas, Ran
AU  - Kormas R
FAU - Solomon, Abraham
AU  - Solomon A
AD  - Department of Ophthalmology, Hadassah-Hebrew University Medical Center, 
      Jerusalem, Israel. dr.avi.solomon@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20140302
PL  - England
TA  - J Inflamm (Lond)
JT  - Journal of inflammation (London, England)
JID - 101232234
PMC - PMC3942779
EDAT- 2014/03/04 06:00
MHDA- 2014/03/04 06:01
PMCR- 2014/03/02
CRDT- 2014/03/04 06:00
PHST- 2013/08/11 00:00 [received]
PHST- 2014/02/17 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/03/04 06:01 [medline]
PHST- 2014/03/02 00:00 [pmc-release]
AID - 1476-9255-11-6 [pii]
AID - 10.1186/1476-9255-11-6 [doi]
PST - epublish
SO  - J Inflamm (Lond). 2014 Mar 2;11(1):6. doi: 10.1186/1476-9255-11-6.