PMID- 24580804
OWN - NLM
STAT- MEDLINE
DCOM- 20150527
LR  - 20220311
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 14
DP  - 2014 Mar 1
TI  - Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of 
      hemorrhagic shock by regulating intestinal blood flow and intestinal expression 
      of p-VASP and ZO-1.
PG  - 80
LID - 10.1186/1472-6882-14-80 [doi]
AB  - BACKGROUND: Dai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long 
      been used clinically in treatment of intestinal obstruction, acute pancreatitis, 
      cholecystalgia and chronic diarrhea for thousands of years. Recent studies have 
      demonstrated that DHFZT can reduce intestinal pathological injury and the 
      concentration of enterogenous endotoxin in an animal model. But the underlying 
      mechanism has not been fully elucidated. METHODS: SD male rats in adult were used 
      to model HS and treated with DHFZT. The serum concentration of endotoxin were 
      analyzed by dynamic turbidimetric method. In addition, the blood flow of small 
      intestine were measured using laser speckle technique. Phosphorylated 
      vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 
      protein, intestinal fatty acid binding protein (IFABP) were measured by Western 
      Blotting, RT-PCR, ELISA respectively. RESULTS: Present study showed that DHFZT 
      markedly elevated the blood flow of small intestine, protected the intestinal 
      barrier function by up-regulating the expression of ZO-1 protein and 
      down-regulating expression of p-VASP, and notely decreased serum concentration of 
      IFABP and endotoxin in HS. CONCLUSIONS: These results reveal that DHFZT improves 
      intestinal blood flow, protects the intestinal barrier function, and ameliorates 
      intestinal endotoxaemia in rats with HS.
FAU - Lu, Xiaoguang
AU  - Lu X
AD  - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 
      116001, China. dllxg@126.com.
FAU - Kang, Xin
AU  - Kang X
FAU - Zhan, Libin
AU  - Zhan L
FAU - Lv, Chunyu
AU  - Lv C
FAU - Fan, Zhiwei
AU  - Fan Z
FAU - Wang, Yingli
AU  - Wang Y
FAU - Ali, Robbie
AU  - Ali R
FAU - Lv, Chang
AU  - Lv C
FAU - Li, Siyao
AU  - Li S
FAU - Mu, Jinhai
AU  - Mu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140301
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 0 (vasodilator-stimulated phosphoprotein)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/*metabolism
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*blood supply/*drug effects
MH  - Male
MH  - Microfilament Proteins/*metabolism
MH  - Phosphoproteins/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regional Blood Flow/*drug effects
MH  - Shock, Hemorrhagic/*drug therapy/*metabolism
MH  - Zonula Occludens-1 Protein/*metabolism
PMC - PMC3974027
EDAT- 2014/03/04 06:00
MHDA- 2015/05/28 06:00
PMCR- 2014/03/01
CRDT- 2014/03/04 06:00
PHST- 2013/11/25 00:00 [received]
PHST- 2014/02/24 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2015/05/28 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - 1472-6882-14-80 [pii]
AID - 10.1186/1472-6882-14-80 [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2014 Mar 1;14:80. doi: 10.1186/1472-6882-14-80.