PMID- 24581515
OWN - NLM
STAT- MEDLINE
DCOM- 20140429
LR  - 20140303
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 83
IP  - 3
DP  - 2014 Mar
TI  - The phytotherapeutic agent, eviprostat, suppresses stromal proliferation and 
      inflammation even after establishment of nonbacterial prostatitis in the rat 
      prostate.
PG  - 528-34
LID - S0090-4295(13)01523-9 [pii]
LID - 10.1016/j.urology.2013.11.033 [doi]
AB  - OBJECTIVE: To evaluate the effect of phytotherapeutic agent, Eviprostat, 
      administered after the establishment of nonbacterial prostatitis (NBP) on the 
      stroma-to-epithelium ratio (S/E ratio), inflammatory scores, tissue macrophage 
      infiltration, and cytokines and chemokines levels in prostate tissue and urine. 
      MATERIALS AND METHODS: Ten-month-old male Wistar rats were castrated and exposed 
      to 17-beta-isomer of estradiol for 30 days to induce NBP. Twenty-five NBP rats 
      were divided into 5 groups: (1) NBP (0) rats sacrificed immediately after the 
      establishment of NBP; (2,3) NBP (30)/control (CTL) and NBP (30)/Eviprostat (EVI) 
      rats fed without or with 0.1% Eviprostat under estradiol-free for 30 days, 
      respectively; and (4,5) NBP (60)/CTL and NBP (60)/EVI rats fed without or with 
      0.1% Eviprostat under estradiol-free for 60 days, respectively. The S/E ratio, 
      inflammatory scores, and the number of macrophage infiltration in the prostate 
      were assessed. Concentrations of cytokines and chemokines in prostatic tissue and 
      urine were measured by enzyme-linked immunosorbent assay. RESULTS: The S/E ratio 
      was significantly increased with time until 60 days under estradiol-free 
      condition (P <.001). The S/E ratio and the inflammatory scores in NBP (60)/EVI 
      was significantly lower than that of NBP (60)/CTL (P <.001, and P = .022, 
      respectively). The mean tissue concentration of chemokine ligand 2 
      (CCL2)/monocyte chemoattractant protein-1 (MCP-1) in NBP (60)/CTL was 
      significantly higher than that in NBP (0) (P = .016), whereas, there was no 
      difference between NBP (60)/EVI and NBP (0). Furthermore, urinary CCL2/MCP-1 was 
      significantly decreased in NBP (60)/EVI as compared with NBP (0) (P = .028). 
      CONCLUSION: Eviprostat suppresses the stromal proliferation and inflammation in 
      the rat prostate after the establishment of NBP at least partly owing to 
      inhibitory effect on CCL2/MCP-1 production in the prostate.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Shibuya, Shinsuke
AU  - Shibuya S
AD  - Department of Diagnostic Pathology, Faculty of Medicine, Kagawa University, 
      Kagawa, Japan.
FAU - Xia, Zhang
AU  - Xia Z
AD  - Department of Urology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
FAU - Sugimoto, Mikio
AU  - Sugimoto M
AD  - Department of Urology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
FAU - Ueda, Nobufumi
AU  - Ueda N
AD  - Department of Urology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
FAU - Haba, Reiji
AU  - Haba R
AD  - Department of Diagnostic Pathology, Faculty of Medicine, Kagawa University, 
      Kagawa, Japan.
FAU - Kakehi, Yoshiyuki
AU  - Kakehi Y
AD  - Department of Urology, Faculty of Medicine, Kagawa University, Kagawa, Japan. 
      Electronic address: kakehi@med.kagawa-u.ac.jp.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines)
RN  - 0 (Drug Combinations)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Plant Extracts)
RN  - 0 (Urological Agents)
RN  - 24YL531VOH (Ethamsylate)
RN  - 4TI98Z838E (Estradiol)
RN  - 59738-67-9 (eviprostat)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/*drug effects
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL3/metabolism
MH  - Chemokine CXCL1/metabolism
MH  - Chemokines/*metabolism/urine
MH  - Disease Models, Animal
MH  - Drug Combinations
MH  - Epithelial Cells/drug effects
MH  - Estradiol
MH  - Ethamsylate/*pharmacology
MH  - Interleukin-1beta/metabolism
MH  - Macrophages
MH  - Male
MH  - Orchiectomy
MH  - Plant Extracts/*pharmacology
MH  - Prostate/drug effects/metabolism/*pathology
MH  - Prostatitis/*drug therapy/metabolism/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/drug effects
MH  - Urological Agents/*pharmacology
EDAT- 2014/03/04 06:00
MHDA- 2014/04/30 06:00
CRDT- 2014/03/04 06:00
PHST- 2013/05/13 00:00 [received]
PHST- 2013/11/08 00:00 [revised]
PHST- 2013/11/20 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/04/30 06:00 [medline]
AID - S0090-4295(13)01523-9 [pii]
AID - 10.1016/j.urology.2013.11.033 [doi]
PST - ppublish
SO  - Urology. 2014 Mar;83(3):528-34. doi: 10.1016/j.urology.2013.11.033.