PMID- 24583103
OWN - NLM
STAT- MEDLINE
DCOM- 20141215
LR  - 20140414
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 153
IP  - 2
DP  - 2014 Apr 28
TI  - Therapeutic effects of dichloromethane fraction of Securidaca inappendiculata on 
      adjuvant-induced arthritis in rat.
PG  - 352-8
LID - S0378-8741(14)00116-0 [pii]
LID - 10.1016/j.jep.2014.02.015 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Securidaca inappendiculata (SI) is a traditional 
      antirheumatic medicine used in China. The present study was designed to 
      investigate the therapeutic efficacy of dichloromethane fraction of SI (SID) at 
      three different doses on adjuvant induced arthritis (AA) rats. METHODS: Arthritis 
      severity was evaluated by arthritic score, body weight loss, paw circumference, 
      histological changes and hyperplasia of lymphatic tissues. Serum samples were 
      collected for estimation of superoxide dismutase (SOD), glutathione (GSH), 
      hydroxy radical (OH.), nitric oxide (NO), malondialdehyde (MDA), N-acetyl 
      glucosaminidase (NAG), sialic acid (SA), alkaline phosphatase (ALP), aspartate 
      transaminase (AST) and alanine transaminase (ALT). The levels of GSH, MDA, NAG 
      and SA in liver were also assessed. The levels of interleukin-1 (IL-1), tumor 
      necrosis factor alpha (TNF-alpha), monocyte chemotactic protein 1 (MCP-1) and 
      vascular endothelial growth factor (VEGF) were determined using ELISA method. 
      Another portion of blood was used for total and differential leucocyte counts. 
      RESULTS: Administration with SID (at high dose with 100 mg/kg) significantly 
      ameliorated the AA severity, suggested by the modulatory effects on body weight 
      loss, paw swelling, hyperplasia of lymphatic tissues and synovial membrane, 
      neutrocytosis and lymphocytosis. It also decreased levels of NO, MDA and OH., 
      restored SOD and GSH levels in serum. The abnormal increased levels of AST, ALT, 
      ALP, NAG and SA significantly were reverted (compared with AA rats, P<0.01). A 
      similar result was observed in livers. Levels of IL-1, TNF-alpha, MCP-1 and VEGF were 
      reduced dramatically by SID too. CONCLUSION: The results suggest SID possesses 
      substantial anti-arthritic activity. The therapeutic efficacy may be due to 
      immumodepressive effects, cytokines regulation, increasing membrane stability and 
      antioxidantive activity.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Zuo, Jian
AU  - Zuo J
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, 
      Nanjing, China.
FAU - Xia, Yan
AU  - Xia Y
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, 
      Nanjing, China.
FAU - Li, Xiang
AU  - Li X
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, 
      Nanjing, China. Electronic address: lixiang_8182@163.com.
FAU - Chen, Jian-wei
AU  - Chen JW
AD  - College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, 
      Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20140226
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Plant Extracts)
RN  - 588X2YUY0A (Methylene Chloride)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*drug therapy/pathology
MH  - Male
MH  - Methylene Chloride/isolation & purification/*therapeutic use
MH  - Plant Extracts/isolation & purification/*therapeutic use
MH  - Plant Stems
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Securidaca
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Anti-arthritis
OT  - Freund׳s complete adjuvant (FCA)
OT  - Rheumatoid arthritis (RA)
OT  - Securidaca inappendiculata
EDAT- 2014/03/04 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/03/04 06:00
PHST- 2013/11/12 00:00 [received]
PHST- 2014/02/07 00:00 [revised]
PHST- 2014/02/08 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - S0378-8741(14)00116-0 [pii]
AID - 10.1016/j.jep.2014.02.015 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2014 Apr 28;153(2):352-8. doi: 10.1016/j.jep.2014.02.015. Epub 
      2014 Feb 26.