PMID- 24583143
OWN - NLM
STAT- MEDLINE
DCOM- 20141228
LR  - 20171116
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 20
IP  - 1
DP  - 2014 May
TI  - Modulation of phenotypic and functional maturation of murine bone-marrow-derived 
      dendritic cells (BMDCs) induced by cadmium chloride.
PG  - 131-40
LID - S1567-5769(14)00069-1 [pii]
LID - 10.1016/j.intimp.2014.02.015 [doi]
AB  - Cadmium (Cd(2+)) has been classified as a type I human carcinogen by the 
      International Agency for Research on Cancer. In the present study, we are going 
      to report for the first time on the detailed modulation of phenotypic and 
      functional maturation of murine bone marrow-derived dendritic cells (BMDCs) 
      induced by cadmium chloride. Dendritic cells (DCs) are major modulators in the 
      whole immune system. One dynamic field of research is the manipulation of DCs as 
      pharmacological targets to screen novel biological modifiers for the treatment of 
      tumor, inflammatory and autoimmune disorders. Phenotypic and functional 
      maturation of BMDCs was evaluated by phase-contrast light microscope for primary 
      cultures, flow cytometry (FCM) for important DC markers, reverse-transcriptase 
      PCR and enzyme linked immunosorbent assay (ELISA) for cytokines. Cd(2+)-induced 
      BMDC death was also performed by comet assay. Our results elucidated that Cd(2+) 
      suppressed maturation of BMDCs via changes as reflected by the decreased 
      expression of key surface molecules such as MHCII and CD40, and also by releasing 
      the lower level of IL-12p70. The change of expression of other co-stimulatory 
      molecules such as CD86 and CD80 was not so significant. However, it was found 
      that cadmium promotes releasing a higher level of IL-23 from BMDCs. So from our 
      study, it can be concluded that cadmium may be one of the potent 
      immunosuppressive agents through the blockage of DC maturation and function.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Chakraborty, Kaustav
AU  - Chakraborty K
AD  - Immunology Lab, Department of Zoology, University of Calcutta, Kolkata 700019, 
      West Bengal, India.
FAU - Chatterjee, Soumya
AU  - Chatterjee S
AD  - Immunology Lab, Department of Zoology, University of Calcutta, Kolkata 700019, 
      West Bengal, India.
FAU - Bhattacharyya, Arindam
AU  - Bhattacharyya A
AD  - Immunology Lab, Department of Zoology, University of Calcutta, Kolkata 700019, 
      West Bengal, India. Electronic address: arindam19@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140226
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (CD40 Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-23)
RN  - 187348-17-0 (Interleukin-12)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - CD40 Antigens/immunology
MH  - Cadmium Chloride/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Interleukin-12/immunology
MH  - Interleukin-23/immunology
MH  - Male
MH  - Mice
MH  - Phenotype
OTO - NOTNLM
OT  - Bone-marrow-derived dendritic cells
OT  - Cadmium chloride
OT  - Immune system
OT  - Immunomodulation
OT  - Immunosuppression
OT  - Maturation
EDAT- 2014/03/04 06:00
MHDA- 2014/12/30 06:00
CRDT- 2014/03/04 06:00
PHST- 2013/11/04 00:00 [received]
PHST- 2014/02/13 00:00 [revised]
PHST- 2014/02/13 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/12/30 06:00 [medline]
AID - S1567-5769(14)00069-1 [pii]
AID - 10.1016/j.intimp.2014.02.015 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2014 May;20(1):131-40. doi: 10.1016/j.intimp.2014.02.015. 
      Epub 2014 Feb 26.