PMID- 24583258
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20181202
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 116
DP  - 2014 Apr 1
TI  - Improved double emulsion technology for fabricating autofluorescent microcapsules 
      as novel ultrasonic/fluorescent dual-modality contrast agents.
PG  - 561-7
LID - S0927-7765(14)00039-3 [pii]
LID - 10.1016/j.colsurfb.2014.01.038 [doi]
AB  - The aim of this study is to explore an improved double emulsion technology with 
      in situ reaction of lysine (Lys) and glutaraldehyde (GA) for fabricating 
      autofluorescent Lys-poly(lactic-co-glycolic acid)-GA (Lys-PLGA-GA) microcapsules 
      as novel ultrasonic/fluorescent dual-modality contrast agents. Scanning electron 
      microscope (SEM) and static light scattering (SLS) results show that 80% of the 
      Lys-PLGA-GA microcapsules are larger than 1.0 mum and 90% of them are smaller than 
      8.9 mum. SEM and laser confocal scanning microscope (LCSM) data demonstrate that 
      the structure of our Lys-PLGA-GA microcapsules is hollow. Compared with the FT-IR 
      spectrum of PLGA microcapsules, a new peak at 1,644 cm(-1) in that of Lys-PLGA-GA 
      microcapsules confirms the formed Schiff base in Lys-PLGA-GA microcapsules. LCSM 
      images and fluorescence spectra show that our Lys-PLGA-GA microcapsules exhibit 
      bright and stable autofluorescence without conjugation to any fluorescent agent, 
      which can be ascribed to the n-pi transitions of the CN bonds in the formed Schiff 
      base. Our autofluorescent Lys-PLGA-GA microcapsules might have more wide 
      applications than traditional fluorescent dyes because their excitation and 
      emission spectra are both broad. The fluorescence intensity can also be tuned by 
      the feeding amount of Lys and GA. The MTT assays reveal that the autofluorescent 
      microcapsules are biocompatible. The results of fluorescent imaging in cells and 
      in vitro ultrasonic imaging demonstrate the feasibility of our autofluorescent 
      Lys-PLGA-GA microcapsules as ultrasonic/fluorescent dual-modality contrast 
      agents. This novel ultrasonic/fluorescent dual-modality contrast agent might have 
      potential for a variety of biological and medical applications.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Gong, An
AU  - Gong A
AD  - Key Laboratory of Magnetic Materials and Devices, Chinese Academy of Sciences, & 
      Division of Functional Materials and Nano Devices, Ningbo Institute of Materials 
      Technology & Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, 
      China.
FAU - Ma, Xuehua
AU  - Ma X
AD  - Key Laboratory of Magnetic Materials and Devices, Chinese Academy of Sciences, & 
      Division of Functional Materials and Nano Devices, Ningbo Institute of Materials 
      Technology & Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, 
      China.
FAU - Xiang, Lingchao
AU  - Xiang L
AD  - Key Laboratory of Magnetic Materials and Devices, Chinese Academy of Sciences, & 
      Division of Functional Materials and Nano Devices, Ningbo Institute of Materials 
      Technology & Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, 
      China.
FAU - Ren, Wenzhi
AU  - Ren W
AD  - Key Laboratory of Magnetic Materials and Devices, Chinese Academy of Sciences, & 
      Division of Functional Materials and Nano Devices, Ningbo Institute of Materials 
      Technology & Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, 
      China.
FAU - Shen, Zheyu
AU  - Shen Z
AD  - Key Laboratory of Magnetic Materials and Devices, Chinese Academy of Sciences, & 
      Division of Functional Materials and Nano Devices, Ningbo Institute of Materials 
      Technology & Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, 
      China. Electronic address: shenzheyu@nimte.ac.cn.
FAU - Wu, Aiguo
AU  - Wu A
AD  - Key Laboratory of Magnetic Materials and Devices, Chinese Academy of Sciences, & 
      Division of Functional Materials and Nano Devices, Ningbo Institute of Materials 
      Technology & Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, 
      China. Electronic address: aiguo@nimte.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140201
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Biocompatible Materials)
RN  - 0 (Capsules)
RN  - 0 (Contrast Media)
RN  - 0 (Emulsions)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - K3Z4F929H6 (Lysine)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Biocompatible Materials/chemical synthesis/chemistry
MH  - Capsules/chemistry
MH  - Cell Survival
MH  - Contrast Media/chemical synthesis/*chemistry
MH  - Emulsions/chemistry
MH  - *Fluorescence
MH  - Glutaral/*chemistry
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Lysine/*chemistry
MH  - MCF-7 Cells
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Surface Properties
MH  - Tumor Cells, Cultured
MH  - *Ultrasonics
OTO - NOTNLM
OT  - Autofluorescent microcapsules
OT  - Double emulsion technology
OT  - Poly(lactic-co-glycolic acid)
OT  - Schiff base
OT  - Ultrasonic/fluorescent dual-modality contrast agents
EDAT- 2014/03/04 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/04 06:00
PHST- 2013/11/27 00:00 [received]
PHST- 2014/01/25 00:00 [revised]
PHST- 2014/01/26 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0927-7765(14)00039-3 [pii]
AID - 10.1016/j.colsurfb.2014.01.038 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2014 Apr 1;116:561-7. doi: 
      10.1016/j.colsurfb.2014.01.038. Epub 2014 Feb 1.