PMID- 24583909
OWN - NLM
STAT- MEDLINE
DCOM- 20141117
LR  - 20140325
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 94
DP  - 2014 Jun
TI  - Rapid extraction and determination of amphetamines in human urine samples using 
      dispersive liquid-liquid microextraction and solidification of floating organic 
      drop followed by high performance liquid chromatography.
PG  - 145-51
LID - S0731-7085(14)00081-8 [pii]
LID - 10.1016/j.jpba.2014.01.044 [doi]
AB  - A novel, rapid, simple and sensitive dispersive liquid-liquid microextraction 
      method based on the solidification of floating organic drop (DLLME-SFO) combined 
      with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was 
      used to determine amphetamine and methamphetamine in urine samples. The factors 
      affecting the extraction efficiency of DLLME-SFO such as the kind and volume of 
      the extraction and the disperser solvents, effect of concentration of K2CO3 and 
      extraction time were investigated and the optimal extraction conditions were 
      established. Under the optimum conditions (extraction solvent: 30.0mul 
      1-undecanol; disperser solvent: 300mul acetonitrile; buffer concentration: 2% 
      (w/v) K2CO3 and extraction time: 1min), calibration curves are linear in the 
      range of 10-3000mugl(-1) and limit of detections (LODs) are in the range of 
      2-8mugl(-1). The relative standard deviations (RSDs) for 100mugl(-1) of amphetamine 
      and methamphetamine in diluted urine are in the range of 6.2-7.8% (n=7). The 
      method was successfully applied for the determination of amphetamine and 
      methamphetamine in the actual urine samples. The relative recoveries of urine 
      samples spiked with amphetamine and methamphetamine are 87.8-113.2%. The obtained 
      results show that DLLME-SFO combined with HPLC-UV is a fast and simple method for 
      the determination of amphetamine and methamphetamine in urine.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Ahmadi-Jouibari, Toraj
AU  - Ahmadi-Jouibari T
AD  - Iran West Center for Drug Abuse Studies, Kermanshah University of Medical 
      Sciences, Kermanshah, Iran.
FAU - Fattahi, Nazir
AU  - Fattahi N
AD  - Research Center for Environmental Determinants of Health (RCEDH), Kermanshah 
      University of Medical Sciences, Kermanshah, Iran. Electronic address: 
      nazirfatahi@yahoo.com.
FAU - Shamsipur, Mojtaba
AU  - Shamsipur M
AD  - Department Chemistry, Razi University, Kermanshah, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140205
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Amphetamines)
RN  - 0 (Solvents)
RN  - 44RAL3456C (Methamphetamine)
SB  - IM
MH  - Amphetamines/*chemistry/*urine
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/methods
MH  - Humans
MH  - Limit of Detection
MH  - Liquid Phase Microextraction/methods
MH  - Methamphetamine/chemistry/urine
MH  - Solvents/chemistry
OTO - NOTNLM
OT  - Amphetamines
OT  - Dispersive liquid-liquid microextraction
OT  - Solidification of floating organic drop
OT  - Urine analysis
EDAT- 2014/03/04 06:00
MHDA- 2014/11/18 06:00
CRDT- 2014/03/04 06:00
PHST- 2014/01/11 00:00 [received]
PHST- 2014/01/26 00:00 [revised]
PHST- 2014/01/29 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/11/18 06:00 [medline]
AID - S0731-7085(14)00081-8 [pii]
AID - 10.1016/j.jpba.2014.01.044 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2014 Jun;94:145-51. doi: 10.1016/j.jpba.2014.01.044. Epub 
      2014 Feb 5.