PMID- 24584758
OWN - NLM
STAT- MEDLINE
DCOM- 20150709
LR  - 20220317
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 20
IP  - 11
DP  - 2014 Nov
TI  - Effects of 5-hydroxymethyl furfural extracted from Rehmannia glutinosa Libosch on 
      the expression of signaling molecules relevant to learning and memory among 
      hippocampal neurons exposed to high concentration of corticosterone.
PG  - 844-9
LID - 10.1007/s11655-014-1830-6 [doi]
AB  - OBJECTIVE: To determine the effects of 5-hydroxymethyl furfural (5-HMF), an 
      extract of Rehmannia glutinosa Libosch, on several down-regulated signaling 
      molecules involved in learning and memory in hippocampal neurons. METHODS: After 
      cultured for 7 days, primary hippocampal neurons were divided into 5 groups: 
      normal, corticosterone model, RU38486, 5-HMF, and donepezil group. Neuron 
      survival rates were calculated 24 h later using SYTO13-PI double-fluorescence 
      staining and an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 
      (MTT) assay. beta-galactosidase activity was also assayed. Protein expressed by the 
      glucocorticoid receptor (GCR), brainderived neurotrophic factor (BDNF), and 
      N-methyl-D-aspartate receptor 2B (NR2B), as well as phosphorylationcyclic 
      adenosine monophosphate (cAMP) response element binding protein (p-CREB), 
      phosphorylation-extracellular signal-regulated kinase (p-ERK), and 
      phosphorylation-synapsin (p-synapsin) were quantified with Western blot. RESULTS: 
      Hippocampal neuron survival rates and the above-mentioned proteins were 
      dramatically decreased (P<0.05), beta-galactosidase activity was significantly 
      increased in the model group. but the effect was reversed by 5-HMF, RU38486, and 
      to a lesser extent by donepezil (P<0.05). CONCLUSION: 5-HMF extracts from the 
      Chinese herb Rehmannia glutinosa Libosch could protect hippocampal neurons from 
      glucocorticoid injury and from down-regulated signaling molecules in the 
      GCR-BDNF-NR2B-p-ERK-p-CREB-p-synapsin signal transduction pathway.
FAU - Zhang, Li-Na
AU  - Zhang LN
AD  - Department of Biochemistry, Institute of Basic Medicine, Shanghai University of 
      Traditional Chinese Medicine, Shanghai, 201203, China.
FAU - Jin, Guo-Qin
AU  - Jin GQ
FAU - Zhang, Xue-Li
AU  - Zhang XL
FAU - Gong, Zhang-Bin
AU  - Gong ZB
FAU - Gu, Cui-Ying
AU  - Gu CY
LA  - eng
PT  - Journal Article
DEP - 20140302
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 70ETD81LF0 (5-hydroxymethylfurfural)
RN  - DJ1HGI319P (Furaldehyde)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Corticosterone/*pharmacology
MH  - Furaldehyde/*analogs & derivatives/isolation & purification/pharmacology
MH  - Hippocampus/cytology/*drug effects
MH  - Learning/*drug effects
MH  - Memory/*drug effects
MH  - Neurons/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rehmannia/*chemistry
MH  - Signal Transduction/*drug effects
EDAT- 2014/03/04 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/03/04 06:00
PHST- 2013/07/03 00:00 [received]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1007/s11655-014-1830-6 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2014 Nov;20(11):844-9. doi: 10.1007/s11655-014-1830-6. Epub 
      2014 Mar 2.