PMID- 24586666
OWN - NLM
STAT- MEDLINE
DCOM- 20150113
LR  - 20220316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 2
DP  - 2014
TI  - Store-operated Ca2+ entry (SOCE) regulates melanoma proliferation and cell 
      migration.
PG  - e89292
LID - 10.1371/journal.pone.0089292 [doi]
LID - e89292
AB  - Store-operated Ca(2+) entry (SOCE) is a major mechanism of Ca(2) (+) import from 
      extracellular to intracellular space, involving detection of Ca(2+) store 
      depletion in endoplasmic reticulum (ER) by stromal interaction molecule (STIM) 
      proteins, which then translocate to plasma membrane and activate Orai Ca(2+) 
      channels there. We found that STIM1 and Orai1 isoforms were abundantly expressed 
      in human melanoma tissues and multiple melanoma/melanocyte cell lines. We 
      confirmed that these cell lines exhibited SOCE, which was inhibited by knockdown 
      of STIM1 or Orai1, or by a pharmacological SOCE inhibitor. Inhibition of SOCE 
      suppressed melanoma cell proliferation and migration/metastasis. Induction of 
      SOCE was associated with activation of extracellular-signal-regulated kinase 
      (ERK), and was inhibited by inhibitors of calmodulin kinase II (CaMKII) or Raf-1, 
      suggesting that SOCE-mediated cellular functions are controlled via the 
      CaMKII/Raf-1/ERK signaling pathway. Our findings indicate that SOCE contributes 
      to melanoma progression, and therefore may be a new potential target for 
      treatment of melanoma, irrespective of whether or not Braf mutation is present.
FAU - Umemura, Masanari
AU  - Umemura M
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Baljinnyam, Erdene
AU  - Baljinnyam E
AD  - Department of Cell Biology and Molecular Medicine, New Jersey Medical School, 
      Rutgers, The State University of New Jersey, Newark, New Jersey, United States of 
      America.
FAU - Feske, Stefan
AU  - Feske S
AD  - Department of Pathology, New York University School of Medicine, New York, New 
      York, United States of America.
FAU - De Lorenzo, Mariana S
AU  - De Lorenzo MS
AD  - Department of Cell Biology and Molecular Medicine, New Jersey Medical School, 
      Rutgers, The State University of New Jersey, Newark, New Jersey, United States of 
      America.
FAU - Xie, Lai-Hua
AU  - Xie LH
AD  - Department of Cell Biology and Molecular Medicine, New Jersey Medical School, 
      Rutgers, The State University of New Jersey, Newark, New Jersey, United States of 
      America.
FAU - Feng, Xianfeng
AU  - Feng X
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Oda, Kayoko
AU  - Oda K
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Makino, Ayako
AU  - Makino A
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Fujita, Takayuki
AU  - Fujita T
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Yokoyama, Utako
AU  - Yokoyama U
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Iwatsubo, Mizuka
AU  - Iwatsubo M
AD  - Department of Cell Biology and Molecular Medicine, New Jersey Medical School, 
      Rutgers, The State University of New Jersey, Newark, New Jersey, United States of 
      America.
FAU - Chen, Suzie
AU  - Chen S
AD  - Department of Chemical Biology, Susan Lehman Cullen Laboratory of Cancer Research 
      in the Ernest Mario School of Pharmacy, Rutgers, The State University of New 
      Jersey, Piscataway, New Jersey, United States of America.
FAU - Goydos, James S
AU  - Goydos JS
AD  - Division of Surgical Oncology, Department of Surgery, Robert Wood Johnson Medical 
      School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, 
      United States of America.
FAU - Ishikawa, Yoshihiro
AU  - Ishikawa Y
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan.
FAU - Iwatsubo, Kousaku
AU  - Iwatsubo K
AD  - Cardiovascular Research Institute, Yokohama City University School of Medicine, 
      Yokohama, Japan ; Department of Cell Biology and Molecular Medicine, New Jersey 
      Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, 
      United States of America.
LA  - eng
GR  - R01 AI097302/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140221
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Calcium Channels)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Channels/metabolism
MH  - Calcium Signaling/*physiology
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Cell Proliferation/*physiology
MH  - Endoplasmic Reticulum/metabolism
MH  - Humans
MH  - Melanocytes/*metabolism
MH  - Melanoma/*metabolism/pathology
MH  - Mice
MH  - Skin Neoplasms/*metabolism/pathology
PMC - PMC3931742
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/03/04 06:00
MHDA- 2015/01/15 06:00
PMCR- 2014/02/21
CRDT- 2014/03/04 06:00
PHST- 2013/05/29 00:00 [received]
PHST- 2014/01/21 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2015/01/15 06:00 [medline]
PHST- 2014/02/21 00:00 [pmc-release]
AID - PONE-D-13-22172 [pii]
AID - 10.1371/journal.pone.0089292 [doi]
PST - epublish
SO  - PLoS One. 2014 Feb 21;9(2):e89292. doi: 10.1371/journal.pone.0089292. eCollection 
      2014.