PMID- 24587178
OWN - NLM
STAT- MEDLINE
DCOM- 20141016
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 2
DP  - 2014
TI  - Electroacupuncture promotes post-stroke functional recovery via enhancing 
      endogenous neurogenesis in mouse focal cerebral ischemia.
PG  - e90000
LID - 10.1371/journal.pone.0090000 [doi]
LID - e90000
AB  - To investigate the question of whether electroacupuncture (EA) promotes 
      functional recovery via enhancement of proliferation and differentiation of 
      neuronal stem cells (NSCs) in ischemic stroke, EA stimulation with 2 Hz was 
      applied at bilateral acupoints to Baihui (GV20) and Dazhui (GV14) in middle 
      cerebral artery occlusion (MCAO) mice. EA stimulation improved neuromotor 
      function and cognitive ability after ischemic stroke. EA stimulation resulted in 
      an increase in the number of proliferated cells, especially in the subventricular 
      zone (SVZ) of the ipsilateral hemisphere. Although a very limited number of NSCs 
      survived and differentiated into neurons or astrocytes, EA treatment resulted in 
      a significant increase in the number of proliferative cells and differentiated 
      cells in the hippocampus and SVZ of the ipsilateral hemisphere compared to MCAO 
      mice. EA stimulation resulted in significantly increased mRNA expression of 
      brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor 
      (VEGF). Protein levels of these factors were confirmed in the ipsilateral 
      hippocampus and SVZ by immunohistochemical and Western blotting analyses. 
      Expression of phosphorylated phosphatidylinositol-3-kinase, BDNF, and 
      VEGF-mediated down-stream were enhanced by EA stimulation in newly formed 
      neuroblasts. These results indicate that EA treatment after ischemic stroke may 
      promote post-stroke functional recovery by enhancement of proliferation and 
      differentiation of NSCs via the BDNF and VEGF signaling pathway.
FAU - Kim, Yu Ri
AU  - Kim YR
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Gyeongnam, Yangsan, Republic of Korea.
FAU - Kim, Ha Neui
AU  - Kim HN
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Gyeongnam, Yangsan, Republic of Korea.
FAU - Ahn, Sung Min
AU  - Ahn SM
AD  - Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan 
      National University, Gyeongnam, Yangsan, Republic of Korea.
FAU - Choi, Yung Hyun
AU  - Choi YH
AD  - Department of Biochemistry, College of Oriental Medicine, Dongeui University, 
      Busan, Yangjeong, Republic of Korea.
FAU - Shin, Hwa Kyoung
AU  - Shin HK
AD  - Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan 
      National University, Gyeongnam, Yangsan, Republic of Korea.
FAU - Choi, Byung Tae
AU  - Choi BT
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Gyeongnam, Yangsan, Republic of Korea ; Division of Meridian and 
      Structural Medicine, School of Korean Medicine, Pusan National University, 
      Gyeongnam, Yangsan, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140224
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Brain/metabolism/pathology/physiopathology
MH  - Brain Ischemia/complications/*pathology/*physiopathology/therapy
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - *Electroacupuncture
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Growth Factors/genetics
MH  - Neural Stem Cells/pathology
MH  - *Neurogenesis
MH  - *Recovery of Function
MH  - Stroke/*complications
PMC - PMC3933702
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/03/04 06:00
MHDA- 2014/10/17 06:00
PMCR- 2014/02/24
CRDT- 2014/03/04 06:00
PHST- 2013/10/18 00:00 [received]
PHST- 2014/01/29 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/10/17 06:00 [medline]
PHST- 2014/02/24 00:00 [pmc-release]
AID - PONE-D-13-45461 [pii]
AID - 10.1371/journal.pone.0090000 [doi]
PST - epublish
SO  - PLoS One. 2014 Feb 24;9(2):e90000. doi: 10.1371/journal.pone.0090000. eCollection 
      2014.