PMID- 24587999
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR  - 20221219
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - Immunomodulation in human dendritic cells leads to induction of interferon-gamma 
      production by Leishmania donovani derived KMP-11 antigen via activation of NF-kappaB 
      in Indian kala-azar patients.
PG  - 947606
LID - 10.1155/2014/947606 [doi]
LID - 947606
AB  - Dendritic cells (DCs) and macrophages (MPhis) are well-known antigen presenting 
      cells with an ability to produce IL-12 which indicates that they have potential 
      of directing acquired immunity toward a Th1-biased response. The aim of this 
      study was to examine the effect of Leishmania specific KMP-11 antigen through 
      comparison of immune responses after presentation by DCs and MPhis to T cells in 
      Indian patients with VL. Patients with DCS and MPhis were directed against a 
      purified Leishmania donovani antigen (KMP-11) and phytohaemagglutinin (PHA). The 
      cytokines (IL-12, IL-10, and TGF-beta) producing abilities of the DCs and MPhis 
      against these antigens were determined by flow cytometry. The transcription 
      factor (NF-kappaB) and T-cell cytokine support (IFN-gamma, IL-10), which could be 
      significant in effector immune function, were also determined. Severe hindrance 
      in the immune protection due to Leishmania parasites, as revealed by decreased 
      expression of IL-12 and upregulation of IL-10 and TGF-beta expression in the MPhis 
      compared to DCs, occurred in VL patients. The production of IL-12 in response to 
      L. donovani KMP-11 antigen was increased in DCs which was reduced in MPhis of VL 
      patients. In contrast, the presentation of KMP-11 antigen by DCs to T-lymphocytes 
      in VL patients significantly increased the IFN-gamma produced by these immune cells, 
      whereas the levels of IL-10 were significantly elevated after presentation of 
      KMP-11 antigen by MPhis. The VL patients were observed with severely dysfunctional 
      MPhis in terms of NF-kappaB activity that could be recovered only after stimulation of 
      DCs with L. donovani KMP-11 antigen. Immunologically the better competitiveness 
      of KMP-11 antigen through a dendritic cell delivery system may be used to revert 
      T-cell anergy, and control strategy can be designed accordingly against 
      kala-azar.
FAU - Chaudhary, Rajesh
AU  - Chaudhary R
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Amit, Ajay
AU  - Amit A
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Yadav, Anupam
AU  - Yadav A
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Singh, Anurag
AU  - Singh A
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Kumar, Vikash
AU  - Kumar V
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Singh, S K
AU  - Singh SK
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Narayan, Shyam
AU  - Narayan S
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Rabidas, Vidyanand
AU  - Rabidas V
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Pandey, K
AU  - Pandey K
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Kumar, Anil
AU  - Kumar A
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Das, Pradeep
AU  - Das P
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India.
FAU - Bimal, Sanjiva
AU  - Bimal S
AD  - Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar 
      800007, India ; Division of Immunology, Rajendra Memorial Research Institute of 
      Medical Sciences, Agamkuan, Patna, Bihar 800007, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20140122
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Protozoan Proteins)
RN  - 0 (kinetoplastid membrane protein 11, Leishmania donovani)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
RIN - Biomed Res Int. 2022 Dec 7;2022:9854346. PMID: 36531649
MH  - Adolescent
MH  - Adult
MH  - Dendritic Cells/immunology/metabolism
MH  - Humans
MH  - Immunomodulation
MH  - Interferon-gamma/*biosynthesis/immunology
MH  - Leishmania donovani/immunology/metabolism/pathogenicity
MH  - Leishmaniasis, Visceral/*genetics/immunology/parasitology
MH  - Male
MH  - Membrane Glycoproteins/immunology/*metabolism
MH  - Middle Aged
MH  - NF-kappa B/*biosynthesis/genetics
MH  - Protozoan Proteins/immunology/*metabolism
MH  - T-Lymphocytes/immunology/metabolism
PMC - PMC3919100
EDAT- 2014/03/04 06:00
MHDA- 2014/10/16 06:00
PMCR- 2014/01/22
CRDT- 2014/03/04 06:00
PHST- 2013/09/16 00:00 [received]
PHST- 2013/12/12 00:00 [revised]
PHST- 2013/12/17 00:00 [accepted]
PHST- 2014/03/04 06:00 [entrez]
PHST- 2014/03/04 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
PHST- 2014/01/22 00:00 [pmc-release]
AID - 10.1155/2014/947606 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:947606. doi: 10.1155/2014/947606. Epub 2014 Jan 22.