PMID- 24588510
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20211021
IS  - 1469-7580 (Electronic)
IS  - 0021-8782 (Print)
IS  - 0021-8782 (Linking)
VI  - 224
IP  - 5
DP  - 2014 May
TI  - Localization of BDNF expression in the developing brain of zebrafish.
PG  - 564-74
LID - 10.1111/joa.12168 [doi]
AB  - The brain-derived neurotrophic factor (BDNF) gene is expressed in differentiating 
      and post-mitotic neurons of the zebrafish embryo, where it has been implicated in 
      Huntington's disease. Little is known, however, about the full complement of 
      neuronal cell types that express BDNF in this important vertebrate model. Here, 
      we further explored the transcriptional profiles during the first week of 
      development using real-time quantitative polymerase chain reaction (RT-qPCR) and 
      whole-mount in situ hybridization (WISH). RT-qPCR results revealed a high level 
      of maternal contribution followed by a steady increase of zygotic transcription, 
      consistent with the notion of a prominent role of BDNF in neuronal maturation and 
      maintenance. Based on WISH, we demonstrate for the first time that BDNF 
      expression in the developing brain of zebrafish is structure specific. Anatomical 
      criteria and co-staining with genetic markers (shh, pax2a, emx1, krox20, lhx2b 
      and lhx9) visualized major topological domains of BDNF-positive cells in the 
      pallium, hypothalamus, posterior tuberculum and optic tectum. Moreover, the 
      relative timing of BDNF transcription in the eye and tectum may illustrate a 
      mechanism for coordinated development of the retinotectal system. Taken together, 
      our results are compatible with a local delivery and early role of BDNF in the 
      developing brain of zebrafish, adding basic knowledge to the study of 
      neurotrophin functions in neural development and disease.
CI  - (c) 2014 Anatomical Society.
FAU - De Felice, E
AU  - De Felice E
AD  - Laboratory of Cellular and Developmental Biology, Stazione Zoologica Anton Dohrn, 
      Naples, Italy; Department of Morphology, Biochemistry, Physiology and Animal 
      Productions, Section of Morphology, University of Messina, Messina, Italy.
FAU - Porreca, I
AU  - Porreca I
FAU - Alleva, E
AU  - Alleva E
FAU - De Girolamo, P
AU  - De Girolamo P
FAU - Ambrosino, C
AU  - Ambrosino C
FAU - Ciriaco, E
AU  - Ciriaco E
FAU - Germana, A
AU  - Germana A
FAU - Sordino, P
AU  - Sordino P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140304
PL  - England
TA  - J Anat
JT  - Journal of anatomy
JID - 0137162
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain/embryology/growth & development/*metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Gene Expression Regulation, Developmental
MH  - In Situ Hybridization
MH  - Neurons/metabolism
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Zebrafish
PMC - PMC3981499
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - central nervous system
OT  - development
OT  - real-time quantitative polymerase chain reaction
OT  - whole-mount in situ hybridization
OT  - zebrafish
EDAT- 2014/03/05 06:00
MHDA- 2014/11/19 06:00
PMCR- 2016/05/01
CRDT- 2014/03/05 06:00
PHST- 2014/01/27 00:00 [accepted]
PHST- 2014/03/05 06:00 [entrez]
PHST- 2014/03/05 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
PHST- 2016/05/01 00:00 [pmc-release]
AID - 10.1111/joa.12168 [doi]
PST - ppublish
SO  - J Anat. 2014 May;224(5):564-74. doi: 10.1111/joa.12168. Epub 2014 Mar 4.