PMID- 24591478
OWN - NLM
STAT- MEDLINE
DCOM- 20140701
LR  - 20140428
IS  - 2042-7670 (Electronic)
IS  - 0042-4900 (Linking)
VI  - 174
IP  - 17
DP  - 2014 Apr 26
TI  - Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2-associated 
      diseases in Croatia.
PG  - 431
LID - 10.1136/vr.102064 [doi]
AB  - Porcine circovirus type 2 (PCV2) causes some of the most significant economic 
      losses in pig production. Several multisystemic syndromes have been attributed to 
      PCV2 infection, which are known as PCV2-associated diseases (PCVDs). This study 
      investigated the origin and evolution of PCV2 sequences in domestic pigs and wild 
      boars affected by PCVDs in Croatia. Viral sequences were recovered from three 
      wild boars diagnosed with PCV2-systemic disease (PCV2-SD), 63 fetuses positive 
      for PCV2 DNA as determined by PCR, 14 domestic pigs affected with PCV2-SD 
      (displaying severe interstitial nephritis) and five domestic pigs with 
      proliferative and necrotising pneumonia. Seventeen complete PCV2 genomes were 
      recovered. Phylogenetic and evolutionary analyses based on median-joining 
      phylogenetic networks, amino acid alignments and principal coordinate analysis 
      were performed using complete genomes, as well as complete and partial ORF 
      sequences for ORF1 and ORF2. Two of the 17 PCV2 sequences belonged to PCV2a, 14 
      to PCV2b and one was unclustered. PCV2b was the predominant genotype in Croatia 
      and has been linked to international trade as a route of introduction. 
      Correlation between particular viral strains with PCVDs is lacking.
FAU - Novosel, D
AU  - Novosel D
AD  - Department of Pathology, Croatian Veterinary Institute, Zagreb 10000, Croatia.
FAU - Tuboly, T
AU  - Tuboly T
FAU - Csagola, A
AU  - Csagola A
FAU - Lorincz, M
AU  - Lorincz M
FAU - Cubric-Curik, V
AU  - Cubric-Curik V
FAU - Jungic, A
AU  - Jungic A
FAU - Curik, I
AU  - Curik I
FAU - Segales, J
AU  - Segales J
FAU - Cortey, M
AU  - Cortey M
FAU - Lipej, Z
AU  - Lipej Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140303
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/*veterinary/virology
MH  - Circovirus/*genetics
MH  - Croatia
MH  - DNA, Viral/genetics
MH  - Genome, Viral
MH  - *Phylogeny
MH  - Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Swine Diseases/*virology
OTO - NOTNLM
OT  - Phylogenetic analysis
OT  - Porcine circovirus type 2
OT  - Porcine circovirus-associated disease
EDAT- 2014/03/05 06:00
MHDA- 2014/07/02 06:00
CRDT- 2014/03/05 06:00
PHST- 2014/03/05 06:00 [entrez]
PHST- 2014/03/05 06:00 [pubmed]
PHST- 2014/07/02 06:00 [medline]
AID - vr.102064 [pii]
AID - 10.1136/vr.102064 [doi]
PST - ppublish
SO  - Vet Rec. 2014 Apr 26;174(17):431. doi: 10.1136/vr.102064. Epub 2014 Mar 3.