PMID- 24593966
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20140407
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 82
DP  - 2014 May
TI  - Reactivity of IgE to the allergen hyaluronidase from Polybia paulista 
      (Hymenoptera, Vespidae) venom.
PG  - 104-11
LID - S0041-0101(14)00060-9 [pii]
LID - 10.1016/j.toxicon.2014.02.016 [doi]
AB  - To date, there are no allergenic extracts or components available in Brazil to 
      diagnosis and treatment of patients with venom allergy from social wasp (Vespidae 
      Family; Polistinae Subfamily) despite of the great number of existing species. We 
      evaluated the immunogenic potential of the Hyal recombinant protein (Pp-Hyal-rec) 
      which was expressed in an insoluble form in comparison with the allergenic native 
      protein (Pp-Hyal-nat) for recognition of immunoglobulin E (IgE) in the serum of 
      allergic patients to venom of the endemic social wasp Polybia paulista from Sao 
      Paulo State, Brazil. Hyal cDNA from the venom of the social wasp P. paulista 
      (Pp-Hyal) (GI: 302201582) was cloned into the expression vector pET-28a in 
      Escherichia coli DE3 (BL21) cells. Solubilization and purification of Pp-Hyal-rec 
      from inclusion bodies were performed using Ni(2+) affinity chromatography 
      (Ni-NTA-Agarose) under denaturing conditions. Both the native (Pp-Hyal-nat) and 
      the recombinant (Pp-Hyal-rec) purified allergens were used for Western blotting 
      to assess the levels of Pp-Hyal-IgE specific in the serum of 10 patients 
      exclusively reactive to the venom of the social wasp P. paulista. The immune sera 
      specifically recognized the band corresponding to the Pp-Hyal-rec protein 
      (40 kDa) at a higher intensity than the native allergen (39 kDa). The sera 
      recognized other proteins in P. paulista crude venom extract to a lesser extent, 
      likely corresponding to other venom allergens such as phospholipase (34 kDa), 
      Antigen 5 (25 kDa), and proteases. The recognition pattern of the immune sera to 
      the Pp-Hyal-rec allergen strongly suggests that this recombinant antigen could be 
      used for developing a diagnostic allergy test as well as for specific 
      immunotherapy (IT).
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Justo Jacomini, Debora Lais
AU  - Justo Jacomini DL
AD  - Laboratorio de Biologia Molecular de Artropodes-LBMA-IB-RC-UNESP (Univ Estadual 
      Paulista), Av. 24-A, n degrees  1515, CEP 13506-900, Bela Vista, Rio Claro, SP, Brazil.
FAU - Gomes Moreira, Susana Margarida
AU  - Gomes Moreira SM
AD  - Centro de Biociencias, Universidade Federal do Rio Grande do Norte, Av. Sen. 
      Salgado Filho, 3000, CEP 59072-970, Candelaria, Natal, RN, Brazil. Electronic 
      address: ssmargarida@gmail.com.
FAU - Campos Pereira, Franco Dani
AU  - Campos Pereira FD
AD  - Laboratorio de Biologia Molecular de Artropodes-LBMA-IB-RC-UNESP (Univ Estadual 
      Paulista), Av. 24-A, n degrees  1515, CEP 13506-900, Bela Vista, Rio Claro, SP, Brazil.
FAU - Zollner, Ricardo de Lima
AU  - Zollner Rde L
AD  - Laboratorio de Imunologia e Alergia Experimental-LIAE, Departamento de Clinica 
      Medica, Faculdade de Ciencias Medicas, FCM, Universidade Estadual de 
      Campinas-UNICAMP, Rua Tessalia Vieira de Camargo n degrees  126, Cidade Universitaria 
      "Zeferino Vaz", CEP 13083-887 Campinas, SP, Brazil. Electronic address: 
      zollner@unicamp.br.
FAU - Brochetto Braga, Marcia Regina
AU  - Brochetto Braga MR
AD  - Laboratorio de Biologia Molecular de Artropodes-LBMA-IB-RC-UNESP (Univ Estadual 
      Paulista), Av. 24-A, n degrees  1515, CEP 13506-900, Bela Vista, Rio Claro, SP, Brazil; 
      Centro de Estudos de Venenos e Animais Peconhentos-CEVAP (Univ Estadual 
      Paulista), Rua Jose Barbosa de Barros, 1780, Fazenda Experimental Lageado, CEP 
      18610-307 Botucatu, SP, Brazil. Electronic address: mrbbraga@rc.unesp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140301
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Allergens)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Wasp Venoms)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
SB  - IM
MH  - Allergens/*genetics/*immunology
MH  - Animals
MH  - Antibody Specificity
MH  - Cloning, Molecular
MH  - Cross Reactions
MH  - Humans
MH  - Hyaluronoglucosaminidase/*immunology
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/*immunology
MH  - Inclusion Bodies/immunology
MH  - Recombinant Proteins/biosynthesis/immunology
MH  - Wasp Venoms/*enzymology/genetics/*immunology
MH  - Wasps/*immunology
OTO - NOTNLM
OT  - Allergen
OT  - Cross-immunoreactivity
OT  - Immunoglobulin E (IgE)
OT  - Polybia paulista
OT  - Recombinant hyaluronidase
OT  - Venom
EDAT- 2014/03/07 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/06 06:00
PHST- 2013/12/06 00:00 [received]
PHST- 2014/02/15 00:00 [revised]
PHST- 2014/02/20 00:00 [accepted]
PHST- 2014/03/06 06:00 [entrez]
PHST- 2014/03/07 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0041-0101(14)00060-9 [pii]
AID - 10.1016/j.toxicon.2014.02.016 [doi]
PST - ppublish
SO  - Toxicon. 2014 May;82:104-11. doi: 10.1016/j.toxicon.2014.02.016. Epub 2014 Mar 1.