PMID- 24594239
OWN - NLM
STAT- MEDLINE
DCOM- 20150107
LR  - 20140507
IS  - 1873-6971 (Electronic)
IS  - 0367-326X (Linking)
VI  - 95
DP  - 2014 Jun
TI  - Lactones from Ligusticum chuanxiong Hort. reduces atherosclerotic lesions in 
      apoE-deficient mice via inhibiting over expression of NF-kB-dependent adhesion 
      molecules.
PG  - 240-6
LID - S0367-326X(14)00057-4 [pii]
LID - 10.1016/j.fitote.2014.02.012 [doi]
AB  - The present study aims to investigate the anti-atherosclerotic effects of 
      lactones extracted from Ligusticum chuanxiong Hort (LLC) in apoE-deficient mice 
      (ApoE(-/-) mice) and proclaim its underlying mechanisms. Expression of 
      endothelial adhesion molecules and NF-kappaB around the atherosclerotic lesions was 
      detected by immunohistochemistry (IHC). To further validate the mechanism, effect 
      of LLC on the secretion of ICAM-1 and VCAM-1 of human umbilical vein endothelial 
      cells (HUVECs) induced by tumor necrosis factor alpha (TNF-alpha) was measured by ELISA. 
      And the activation of NF-kappaB was detected by western blot. Mice treated with LLC 
      showed significant reduction in lesion sizes of thoracic segments of the aorta 
      (p<0.01). Meanwhile, LLC treatments lead to decreases of serum TG, TC and LDL-C 
      contents, respectively. LLC also decreased the expression of CD31, intercellular 
      adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) and 
      nuclear factor-kappa B (NF-kappaB) in the atherosclerotic plaque. Moreover, LLC at 
      3.125-25 mug/mL can dose-dependently attenuate the expression of ICAM-1 and VCAM-1 
      in TNF-alpha stimulated HUVECs. Western blot result indicated LLC inhibited 
      activation of NF-kappaB. These results suggested that LLC could ameliorate 
      atherosclerosis in ApoE(-/-) mice. The mechanism of action of LLC on 
      anti-atherosclerotic effect may be attributed to the suppression of the 
      production of NF-kappaB-dependent adhesion molecules.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Xiao, Yang
AU  - Xiao Y
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin 300193, China.
FAU - Wang, Ying-Chao
AU  - Wang YC
AD  - Department of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, 
      China.
FAU - Li, Lai-Lai
AU  - Li LL
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin 300193, China.
FAU - Jin, Ye-Cheng
AU  - Jin YC
AD  - Department of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, 
      China.
FAU - Sironi, Luigi
AU  - Sironi L
AD  - Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, 
      China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin 300193, China. Electronic address: 
      vip_wangyi@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140302
PL  - Netherlands
TA  - Fitoterapia
JT  - Fitoterapia
JID - 16930290R
RN  - 0 (Apolipoproteins E)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Lactones)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
EIN - Fitoterapia. 2014 Jul;96:159
MH  - Animals
MH  - Aorta/pathology
MH  - Apolipoproteins E/genetics
MH  - Atherosclerosis/*drug therapy
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Disease Models, Animal
MH  - Down-Regulation/drug effects
MH  - Gene Expression Regulation/*drug effects
MH  - Human Umbilical Vein Endothelial Cells/drug effects/metabolism
MH  - Humans
MH  - Lactones/chemistry/isolation & purification/*therapeutic use
MH  - Ligusticum/*chemistry
MH  - Mice
MH  - Mice, Knockout
MH  - NF-kappa B/*drug effects/metabolism
MH  - Plants, Medicinal
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Adhesion molecule
OT  - ApoE(-/-)
OT  - HUVEC
OT  - Lactones
OT  - NF-kappaB
OT  - TNF-alpha
EDAT- 2014/03/07 06:00
MHDA- 2015/01/08 06:00
CRDT- 2014/03/06 06:00
PHST- 2013/12/23 00:00 [received]
PHST- 2014/02/20 00:00 [revised]
PHST- 2014/02/22 00:00 [accepted]
PHST- 2014/03/06 06:00 [entrez]
PHST- 2014/03/07 06:00 [pubmed]
PHST- 2015/01/08 06:00 [medline]
AID - S0367-326X(14)00057-4 [pii]
AID - 10.1016/j.fitote.2014.02.012 [doi]
PST - ppublish
SO  - Fitoterapia. 2014 Jun;95:240-6. doi: 10.1016/j.fitote.2014.02.012. Epub 2014 Mar 
      2.