PMID- 24594260 OWN - NLM STAT- MEDLINE DCOM- 20150217 LR - 20141203 IS - 1090-2449 (Electronic) IS - 0014-4894 (Linking) VI - 145 Suppl DP - 2014 Nov TI - Endosymbiotic Mycobacterium chelonae in a Vermamoeba vermiformis strain isolated from the nasal mucosa of an HIV patient in Lima, Peru. PG - S127-30 LID - S0014-4894(14)00031-9 [pii] LID - 10.1016/j.exppara.2014.02.014 [doi] AB - In March 2010, a 35 year-old HIV/AIDS female patient was admitted to hospital to start treatment with Highly Active Antiretroviral Therapy (HAART) since during a routine control a dramatic decrease in the CD4(+) levels was detected. At this stage, a nasal swab from each nostril was collected from the patient to include it in the samples for the case study mentioned above. Moreover, it is important to mention that the patient was diagnosed in 2009 with invasive pneumococcal disease, acute cholecystitis, pancreatitis and pulmonary tuberculosis. The collected nasal swabs from both nostrils were positive for Vermamoeba vermiformis species which was identified using morphological and PCR/DNA sequencing approaches. Basic Local Alignment Search Tool (BLAST) homology and phylogenetic analysis confirmed the amoebic strain to belong to V.vermiformis species. Molecular identification of the Mycobacterium strain was carried out using a bacterial universal primer pair for the 16S rDNA gene at the genus level and the rpoB gene was amplified and sequenced as previously described to identify the Mycobacterium species (Shin et al., 2008; Sheen et al., 2013). Homology and phylogenetic analyses of the rpoB gene confirmed the species as Mycobacterium chelonae. In parallel, collected swabs were tested by PCR and were positive for the presence of V.vermiformis and M.chelonae. This work describes the identification of an emerging bacterial pathogen,M.chelonae from a Free-Living Amoebae (FLA) strain belonging to the species V.vermiformis that colonized the nasal cavities of an HIV/AIDS patient, previously diagnosed with TB. Awareness within clinicians and public health professionals should be raised, as pathogenic agents such as M.chelonae may be using FLA to propagate and survive in the environment. CI - Copyright (c) 2014 Elsevier Inc. All rights reserved. FAU - Cabello-Vilchez, Alfonso Martin AU - Cabello-Vilchez AM AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain; Medical Technology School, Universidad Peruana Cayetano Heredia, Lima, Peru. FAU - Mena, Rosmery AU - Mena R AD - Immunohistochemistry Laboratory, National Hospital Cayetano Heredia, Lima, Peru. FAU - Zuniga, Johanna AU - Zuniga J AD - Medical Technology School, Universidad Peruana Cayetano Heredia, Lima, Peru. FAU - Cermeno, Pablo AU - Cermeno P AD - School of Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru. FAU - Martin-Navarro, Carmen Ma AU - Martin-Navarro CM AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain; Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, Edinburgh, Scotland, UK. FAU - Gonzalez, Ana C AU - Gonzalez AC AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain. FAU - Lopez-Arencibia, Atteneri AU - Lopez-Arencibia A AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain. FAU - Reyes-Batlle, Maria AU - Reyes-Batlle M AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain. FAU - Pinero, Jose E AU - Pinero JE AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain. FAU - Valladares, Basilio AU - Valladares B AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain. FAU - Lorenzo-Morales, Jacob AU - Lorenzo-Morales J AD - University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain. Electronic address: jmlorenz@ull.edu.es. LA - eng SI - GENBANK/KF470967 SI - GENBANK/KF471661 PT - Case Reports PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140301 PL - United States TA - Exp Parasitol JT - Experimental parasitology JID - 0370713 RN - 0 (DNA, Bacterial) RN - 0 (DNA, Protozoan) SB - IM MH - Adult MH - Amebiasis/*complications MH - DNA, Bacterial/isolation & purification MH - DNA, Protozoan/isolation & purification MH - Disease Reservoirs MH - Female MH - HIV Infections/*complications/microbiology/parasitology MH - Hartmannella/genetics/isolation & purification/*microbiology MH - Humans MH - Molecular Sequence Data MH - Mycobacterium Infections, Nontuberculous/*complications/transmission MH - Mycobacterium chelonae/genetics/*isolation & purification/physiology MH - Nasal Mucosa/microbiology/parasitology MH - Peru MH - *Symbiosis OTO - NOTNLM OT - Amoebae OT - Mycobacterium chelonae OT - PCR OT - Vermamoeba vermiformis EDAT- 2014/03/07 06:00 MHDA- 2015/02/18 06:00 CRDT- 2014/03/06 06:00 PHST- 2013/12/05 00:00 [received] PHST- 2014/02/06 00:00 [revised] PHST- 2014/02/18 00:00 [accepted] PHST- 2014/03/06 06:00 [entrez] PHST- 2014/03/07 06:00 [pubmed] PHST- 2015/02/18 06:00 [medline] AID - S0014-4894(14)00031-9 [pii] AID - 10.1016/j.exppara.2014.02.014 [doi] PST - ppublish SO - Exp Parasitol. 2014 Nov;145 Suppl:S127-30. doi: 10.1016/j.exppara.2014.02.014. Epub 2014 Mar 1.