PMID- 24595683
OWN - NLM
STAT- MEDLINE
DCOM- 20140811
LR  - 20220318
IS  - 1432-0711 (Electronic)
IS  - 0932-0067 (Linking)
VI  - 290
IP  - 1
DP  - 2014 Jul
TI  - Morphological good-quality embryo has higher nucleus spreading rate/signal 
      resolution rate in fluorescence in situ hybridization.
PG  - 185-90
LID - 10.1007/s00404-014-3189-5 [doi]
AB  - PURPOSE: To evaluate the relationship between day 3 embryo quality and nucleus 
      spreading rate/signal resolution rate in Fluorescence in situ hybridization 
      (FISH) during the PGD procedure. METHODS: This study was a retrospective data 
      analysis. 367 day-3 embryos were classified based on morphological scoring: grade 
      1 to grade 4 were defined from worse to better embryo quality. Day 3 embryos were 
      classified as good quality when the number of blastomeres was between 6 and 10 
      and grade better than 2'. Nucleus spreading rate, signal rate and the full signal 
      rate were compared between embryos with different morphological scoring. RESULTS: 
      Nucleus spreading rate of blastomeres from morphological high-quality embryos was 
      significantly higher (86.25 %) than from poor-quality embryos (76.53 %) (p < 
      0.05). The rate of blastomeres with full signals was significantly higher (79.32 
      %) in the morphological high-quality group than in poor-quality group (64.54 %) 
      (p < 0.05). Similar results were found from day 3 embryos with cell number 
      between 6 cells and 10 cells (nucleus spreading rate 86.01 vs. 76.34 %, p < 0.05; 
      full signal rate 78.72 vs. 62.71 %, p < 0.05). Both have no significant 
      difference in the signal rate (82.67 vs. 89.66 %; 83.10 vs. 89.95 %). 
      CONCLUSIONS: Blastomeres from day 3 embryos with better morphological quality had 
      higher nucleus spreading rate and higher full signal rate during FISH. Through 
      this study, we speculate on whether it should reconsider the necessity of FISH 
      application in embryos with poor quality.
FAU - Wu, Keliang
AU  - Wu K
AD  - Centre for Reproductive Medicine, Provincial Hospital Affiliated to Shandong 
      University, Jinan, 250021, China.
FAU - Zheng, Ye
AU  - Zheng Y
FAU - Zhu, Yueting
AU  - Zhu Y
FAU - Li, Hongchang
AU  - Li H
FAU - Yu, Guanling
AU  - Yu G
FAU - Yan, Junhao
AU  - Yan J
FAU - Chen, Zi-Jiang
AU  - Chen ZJ
LA  - eng
PT  - Journal Article
DEP - 20140305
PL  - Germany
TA  - Arch Gynecol Obstet
JT  - Archives of gynecology and obstetrics
JID - 8710213
SB  - IM
MH  - Blastomeres/*cytology/physiology
MH  - *Cell Nucleus
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nuclear Transfer Techniques
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - Preimplantation Diagnosis/*methods
MH  - Retrospective Studies
EDAT- 2014/03/07 06:00
MHDA- 2014/08/12 06:00
CRDT- 2014/03/06 06:00
PHST- 2013/10/08 00:00 [received]
PHST- 2014/02/13 00:00 [accepted]
PHST- 2014/03/06 06:00 [entrez]
PHST- 2014/03/07 06:00 [pubmed]
PHST- 2014/08/12 06:00 [medline]
AID - 10.1007/s00404-014-3189-5 [doi]
PST - ppublish
SO  - Arch Gynecol Obstet. 2014 Jul;290(1):185-90. doi: 10.1007/s00404-014-3189-5. Epub 
      2014 Mar 5.