PMID- 24595999
OWN - NLM
STAT- MEDLINE
DCOM- 20140605
LR  - 20191112
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
VI  - 23
IP  - 1
DP  - 2014 Jan-Feb
TI  - In vitro effects of Candida albicans and Aspergillus fumigatus on dendritic cells 
      and the role of beta glucan in this effect.
PG  - 17-24
AB  - BACKGROUND: Dendritic cells (DCs) are able to initiate and regulate the immune 
      response to fungal infections. beta-glucan stimulates the immune system, modulating 
      cellular and humoral immunity. It has a beneficial effect in fighting fungal 
      infections. OBJECTIVES: We investigated the in vitro effect of C.albicans and 
      A.fumigatus infection on human DCs. The cytokine levels were determined by ELISA. 
      MATERIAL AND METHODS: Human PBMCs isolation was performed by Ficoll-hypaque 
      density gradient centrifugation method. DCs maturation was analysed by using flow 
      cytometry. The cytokine levels were determined by ELISA. RESULTS: DCs stimulated 
      by C. albicans and A. fumigatus induced DC maturation by increasing CD80 and CD86 
      co-stimulatory molecules. DCs stimulated by fungi produced IL-8 and IL-12p70. 
      Whereas IL-10 production from the stimulated DCs did not differ from uninfected 
      DCs. Also, the addition of beta-glucan to the DCs stimulated by fungi promoted the 
      activation and maturation of DCs. CONCLUSIONS: Our results suggest that DCs are 
      capable of initiating an innate and adaptive immune response against fungal 
      infections. In addition, beta-glucan can be used as a novel stimulator to DC-based 
      vaccination against fungal infections.
FAU - Fidan, Isil
AU  - Fidan I
AD  - Gazi University Faculty of Medicine, Department of Medical Microbiology, Ankara, 
      Turkey.
FAU - Kalkanci, Ayse
AU  - Kalkanci A
FAU - Yesilyurt, Emine
AU  - Yesilyurt E
FAU - Erdal, Berna
AU  - Erdal B
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
RN  - 0 (Interleukin-8)
RN  - 0 (beta-Glucans)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Aspergillus fumigatus/*immunology
MH  - Candida albicans/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*immunology/physiology
MH  - Humans
MH  - Interleukin-12/analysis
MH  - Interleukin-8/analysis
MH  - beta-Glucans/*pharmacology
EDAT- 2014/03/07 06:00
MHDA- 2014/06/06 06:00
CRDT- 2014/03/06 06:00
PHST- 2014/03/06 06:00 [entrez]
PHST- 2014/03/07 06:00 [pubmed]
PHST- 2014/06/06 06:00 [medline]
AID - 10.17219/acem/37016 [doi]
PST - ppublish
SO  - Adv Clin Exp Med. 2014 Jan-Feb;23(1):17-24. doi: 10.17219/acem/37016.