PMID- 24597471
OWN - NLM
STAT- MEDLINE
DCOM- 20141203
LR  - 20231110
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 44
IP  - 5
DP  - 2014
TI  - Increased local IgE production induced by common aeroallergens and phenotypic 
      alteration of mast cells in Chinese eosinophilic, but not non-eosinophilic, 
      chronic rhinosinusitis with nasal polyps.
PG  - 690-700
LID - 10.1111/cea.12304 [doi]
AB  - BACKGROUND: Eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal 
      polyps (CRSwNP) display distinct patterns of inflammation. However, the 
      pathogenic mechanisms underlying the heterogeneity of CRSwNP need further 
      investigation. OBJECTIVE: To investigate local immunoglobulin E (IgE) production 
      and phenotype of mast cells in eosinophilic and non-eosinophilic CRSwNP in 
      Chinese. METHODS: Total and specific IgE levels were analysed by means of the 
      ImmunoCAP system. The molecular steps involved in class-switch recombination to 
      IgE were investigated using RT-PCR assays. Mast cell phenotypes, IgE- and 
      high-affinity IgE receptor (FcepsilonRI)-positive cells, and allergen binding to 
      specific IgE in sinonasal mucosa were determined by means of 
      immunohistochemistry. RESULTS: Compared with controls and non-eosinophilic 
      CRSwNP, local total IgE levels were increased, and local specific IgE to common 
      aeroallergens was more frequently found, in Chinese eosinophilic CRSwNP 
      independent of atopy and without significant association with Staphylococcus 
      aureus enterotoxins. The epsilon germline gene transcript was also more frequently 
      detected in eosinophilic CRSwNP. The number of IgE- and FcepsilonRI-positive cells was 
      increased in eosinophilic CRSwNP. Most IgE- and FcepsilonRI-positive cells were mast 
      cells. Dust mite antigens could bind to IgE on mast cells in situ. The number of 
      mast cells positive for both tryptase and chymase and activated mast cells was 
      increased in eosinophilic CRSwNP and the number of activated mast cells 
      positively correlated with local IgE level, eotaxin-1 level, and eosinophil count 
      in CRSwNP. CONCLUSIONS AND CLINICAL RELEVANCE: The local IgE induced by common 
      aeroallergens may mediate mast cell activation and contribute to subsequent 
      eosinophilic inflammation in Chinese CRSwNP. This study offers a rationale for 
      considering intervention strategies designed to target 'local allergy' in 
      eosinophilic CRSwNP.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Cao, P-P
AU  - Cao PP
AD  - Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji 
      Medical College, Huazhong University of Science and Technology, Wuhan, China.
FAU - Zhang, Y-N
AU  - Zhang YN
FAU - Liao, B
AU  - Liao B
FAU - Ma, J
AU  - Ma J
FAU - Wang, B-F
AU  - Wang BF
FAU - Wang, H
AU  - Wang H
FAU - Zeng, M
AU  - Zeng M
FAU - Liu, W-H
AU  - Liu WH
FAU - Schleimer, R P
AU  - Schleimer RP
FAU - Liu, Z
AU  - Liu Z
LA  - eng
GR  - P01 AI106683/AI/NIAID NIH HHS/United States
GR  - R01 HL078860/HL/NHLBI NIH HHS/United States
GR  - R37HL068546/HL/NHLBI NIH HHS/United States
GR  - R37 HL068546/HL/NHLBI NIH HHS/United States
GR  - R01HL078860/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Air Pollutants)
RN  - 0 (Allergens)
RN  - 0 (Antigens)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgE)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Air Pollutants/immunology
MH  - Allergens/*immunology
MH  - Antigens/immunology
MH  - Asian People
MH  - B-Lymphocyte Subsets/immunology/metabolism
MH  - CD40 Antigens/metabolism
MH  - CD40 Ligand/metabolism
MH  - Chemotaxis, Leukocyte/immunology
MH  - China
MH  - Chronic Disease
MH  - Cytokines/metabolism
MH  - Eosinophilia/genetics/*immunology/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Leukocyte Count
MH  - Male
MH  - Mast Cells/*immunology/metabolism
MH  - Middle Aged
MH  - Nasal Polyps/complications
MH  - Phenotype
MH  - Protein Binding/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, IgE/metabolism
MH  - Rhinitis/complications/diagnosis/genetics/*immunology
MH  - Sinusitis/complications/diagnosis/genetics/*immunology
MH  - Th2 Cells/immunology/metabolism
MH  - Young Adult
PMC - PMC4013492
MID - NIHMS573601
OTO - NOTNLM
OT  - chronic rhinosinusitis
OT  - eosinophil
OT  - immunoglobulin E
OT  - mast cell
OT  - nasal polyps
EDAT- 2014/03/07 06:00
MHDA- 2014/12/15 06:00
PMCR- 2015/05/01
CRDT- 2014/03/07 06:00
PHST- 2013/11/20 00:00 [received]
PHST- 2014/02/24 00:00 [revised]
PHST- 2014/02/24 00:00 [accepted]
PHST- 2014/03/07 06:00 [entrez]
PHST- 2014/03/07 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2015/05/01 00:00 [pmc-release]
AID - 10.1111/cea.12304 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2014;44(5):690-700. doi: 10.1111/cea.12304.