PMID- 24597761
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20220321
IS  - 1756-185X (Electronic)
IS  - 1756-1841 (Linking)
VI  - 19
IP  - 4
DP  - 2016 Apr
TI  - Histopathological changes in supraspinous ligaments, ligamentum flava and 
      paraspinal muscle tissues of patients with ankylosing spondylitis.
PG  - 420-9
LID - 10.1111/1756-185X.12305 [doi]
AB  - OBJECTIVE: To examine the histopathological changes in spinal tissues of 
      ankylosing spondylitis (AS) patients. METHODS: Tissue samples from 10 AS patients 
      and 10 control subjects were obtained. Hematoxylin and eosin, picrosirius, Masson 
      and van Gieson stainings were utilized to determine the pathological changes in 
      tissues. Ultrastructural alterations were examined by electronic microscopy. 
      Proteoglycan levels were assessed by enzyme-linked immunosorbent assays (ELISA). 
      Matrix metalloproteinase-3 (MMP-3), transforming growth factor-beta1 (TGF-beta1) and 
      tumor necrosis factor-alpha (TNF-alpha) levels were evaluated by immunohistochemistry. 
      RESULTS: Our results demonstrate that the density of collagen fibrils was reduced 
      in the supraspinous ligaments of AS tissue and fibrils were loosely and 
      irregularly organized as compared to a regular distribution of collagen fibrils 
      in controls. In ligamentum flava from AS patients, activated fibroblasts with 
      enlarged nuclei were detected, while the number of elastic fibers was greatly 
      decreased. Paraspinal muscle tissues of AS patients exhibited increased collagen 
      fibril accumulation and atrophy. Significantly decreased proteoglycan and 
      elevated MMP-3 levels were found in supraspinous ligament samples from AS 
      patients (P < 0.01). Additionally, the levels of TGF-beta1 in ligamentum flava and 
      paraspinal muscle tissues of AS patients were increased (P < 0.01). The 
      expression of TNF-alpha was also upregulated in the ligamentum flavum (P < 0.01), 
      with no significant difference in the paraspinal muscle between control and AS 
      patients (P > 0.05). CONCLUSIONS: Our findings reveal histopathological changes 
      that occur in certain spinal tissues of AS patients and suggest that increased 
      levels of MMP-3 and TGF-beta1 may contribute to the pathogenesis of AS.
CI  - (c) 2014 The Authors. International Journal of Rheumatic Diseases published by Asia 
      Pacific League of Associations for Rheumatology and Wiley Publishing Asia Pty 
      Ltd.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical 
      University, Chongqing, China.
FAU - Xu, Hongfei
AU  - Xu H
AD  - Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical 
      University, Chongqing, China.
FAU - Hu, Xu
AU  - Hu X
AD  - Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical 
      University, Chongqing, China.
FAU - Zhang, Chao
AU  - Zhang C
AD  - Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical 
      University, Chongqing, China.
FAU - Chu, Tongwei
AU  - Chu T
AD  - Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical 
      University, Chongqing, China.
FAU - Zhou, Yue
AU  - Zhou Y
AD  - Department of Orthopaedics, Xinqiao Hospital, the Third Military Medical 
      University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20140306
PL  - England
TA  - Int J Rheum Dis
JT  - International journal of rheumatic diseases
JID - 101474930
RN  - 0 (Biomarkers)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Proteoglycans)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Biomarkers/analysis
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Inflammation Mediators/analysis
MH  - Ligamentum Flavum/enzymology/*pathology/ultrastructure
MH  - Male
MH  - Matrix Metalloproteinase 3/analysis
MH  - Middle Aged
MH  - Paraspinal Muscles/enzymology/*pathology/ultrastructure
MH  - Proteoglycans/analysis
MH  - Spondylitis, Ankylosing/enzymology/*pathology
MH  - Transforming Growth Factor beta1/*analysis
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Young Adult
OTO - NOTNLM
OT  - MMP-3
OT  - TGF-beta1
OT  - ankylosing spondylitis (AS)
OT  - collagen fibril
OT  - histopathology
EDAT- 2014/03/07 06:00
MHDA- 2017/05/10 06:00
CRDT- 2014/03/07 06:00
PHST- 2014/03/07 06:00 [entrez]
PHST- 2014/03/07 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
AID - 10.1111/1756-185X.12305 [doi]
PST - ppublish
SO  - Int J Rheum Dis. 2016 Apr;19(4):420-9. doi: 10.1111/1756-185X.12305. Epub 2014 
      Mar 6.