PMID- 24604153
OWN - NLM
STAT- MEDLINE
DCOM- 20141208
LR  - 20211021
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 22
IP  - 1
DP  - 2014 Apr
TI  - Comparative cytogenomics of poultry: mapping of single gene and repeat loci in 
      the Japanese quail (Coturnix japonica).
PG  - 71-83
LID - 10.1007/s10577-014-9411-2 [doi]
AB  - Well-characterized molecular and cytogenetic maps are yet to be established in 
      Japanese quail (Coturnix japonica). The aim of the current study was to 
      cytogenetically map and determine linkage of specific genes and gene complexes in 
      Japanese quail through the use of chicken (Gallus gallus) and turkey (Meleagris 
      gallopavo) genomic DNA probes and conduct a comparative study among the three 
      genomes. Chicken and turkey clones were used as probes on mitotic metaphase and 
      meiotic pachytene stage chromosomes of the three species for the purpose of 
      high-resolution fluorescence in situ hybridization (FISH). The genes and 
      complexes studied included telomerase RNA (TR), telomerase reverse transcriptase 
      (TERT), 5S rDNA, 18S-5.8S-28S rDNA (i.e., nucleolus organizer region (NOR)), and 
      the major histocompatibility complex (MHC). The telomeric profile of Japanese 
      quail was investigated through the use of FISH with a TTAGGG-PNA probe. A range 
      of telomeric array sizes were confirmed as found for the other poultry species. 
      Three NOR loci were identified in Japanese quail, and single loci each for TR, 
      TERT, 5S rDNA and the MHC-B. The MHC-B and one NOR locus were linked on a 
      microchromosome in Japanese quail. We confirmed physical linkage of 5S rDNA and 
      the TR gene on an intermediate-sized chromosome in quail, similar to both chicken 
      and turkey. TERT localized to CJA 2 in quail and the orthologous chromosome 
      region in chicken (GGA 2) and in turkey (MGA 3). The cytogenetic profile of 
      Japanese quail was further developed by this study and synteny was identified 
      among the three poultry species.
FAU - McPherson, Marla C
AU  - McPherson MC
AD  - Department of Animal Science, University of California, One Shields Ave, Davis, 
      CA, 95616, USA.
FAU - Robinson, Charmaine M
AU  - Robinson CM
FAU - Gehlen, Lida P
AU  - Gehlen LP
FAU - Delany, Mary E
AU  - Delany ME
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (RNA, Ribosomal)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - Coturnix/*genetics
MH  - Cytogenetic Analysis/methods/*veterinary
MH  - Genetic Linkage/genetics
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Major Histocompatibility Complex/genetics
MH  - Poultry/*genetics
MH  - RNA, Ribosomal/genetics
MH  - Species Specificity
MH  - Telomerase/genetics
MH  - Telomere/*genetics
EDAT- 2014/03/08 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/08 06:00
PHST- 2014/03/08 06:00 [entrez]
PHST- 2014/03/08 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1007/s10577-014-9411-2 [doi]
PST - ppublish
SO  - Chromosome Res. 2014 Apr;22(1):71-83. doi: 10.1007/s10577-014-9411-2.