PMID- 24604573
OWN - NLM
STAT- MEDLINE
DCOM- 20141113
LR  - 20161125
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 44
IP  - 5
DP  - 2014 May
TI  - Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced 
      proliferation, metastasis and lung cancer-associated inflammation in vitro.
PG  - 1561-70
LID - 10.3892/ijo.2014.2320 [doi]
AB  - The objective of the present study was to investigate the therapeutic efficacy of 
      flavonoid components in Scutellaria baicalensis on proliferation, metastasis and 
      lung cancer-associated inflammation during nicotine induction in the A549 and 
      H1299 lung cancer cell lines. After experimental period, augmentation of 
      proliferation was observed, accompanied by marked decrease in apoptotic cells in 
      nicotine-induced lung cancer cells; additionally, nicotine-exposed cells 
      exhibited increased invasive and migratory abilities based on invasion and 
      wound-healing assay. Flavones in Scutellaria, baicalin, baicalein and wogonin 
      significantly counteracted the above deleterious changes. Moreover, assessment of 
      tumor apoptotic and metastatic factors on mRNA levels by quantitative PCR and 
      protein levels by western blotting revealed that these phytochemical treatments 
      effectively negated nicotine-induced upregulated expression of bcl-2, bcl-2/bax 
      ratio, caspase-3, matrix metalloproteinase (MMP)-2 and MMP-9 as well as 
      downregulated expression of bax. Further analysis of inflammatory markers such as 
      tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in cell culture supernatant 
      and mRNA and protein expression of nuclear transcription factor-kappaB (NF-kappaB) 
      and I kappa B-alpha (IkappaB-alpha) was carried out to substantiate the anti-inflammatory 
      effect of flavones in Scutellaria in nicotine-exposed lung cancer cells. The 
      therapeutic effects observed in the present study are attributed to the potent 
      potential against proliferation, metastasis and inflammatory microenvironment by 
      flavonoid components in Scutellaria in nicotine-induced lung cancer cells.
FAU - Gong, Wei-Yi
AU  - Gong WY
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Wu, Jin-Feng
AU  - Wu JF
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Liu, Bao-Jun
AU  - Liu BJ
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Zhang, Hong-Ying
AU  - Zhang HY
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Cao, Yu-Xue
AU  - Cao YX
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Sun, Jing
AU  - Sun J
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Lv, Yu-Bao
AU  - Lv YB
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Wu, Xiao
AU  - Wu X
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
FAU - Dong, Jing-Cheng
AU  - Dong JC
AD  - Institute of Integrative Medicine, Department of Integrative Medicine, Huashan 
      Hospital, Fudan University, Shanghai 200041, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140305
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Flavanones)
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
RN  - 0 (Scutellaria baicalensis extract)
RN  - 347Q89U4M5 (baicalin)
RN  - 49QAH60606 (baicalein)
RN  - 6M3C89ZY6R (Nicotine)
RN  - POK93PO28W (wogonin)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Flavanones/pharmacology
MH  - Flavonoids/*pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Inflammation/chemically induced/complications/*pathology
MH  - Lung Neoplasms/chemically induced/complications/*pathology
MH  - Neoplasm Metastasis/*pathology
MH  - Nicotine/*adverse effects
MH  - Plant Extracts/*pharmacology
MH  - Scutellaria baicalensis
EDAT- 2014/03/08 06:00
MHDA- 2014/11/14 06:00
CRDT- 2014/03/08 06:00
PHST- 2013/12/25 00:00 [received]
PHST- 2014/02/12 00:00 [accepted]
PHST- 2014/03/08 06:00 [entrez]
PHST- 2014/03/08 06:00 [pubmed]
PHST- 2014/11/14 06:00 [medline]
AID - 10.3892/ijo.2014.2320 [doi]
PST - ppublish
SO  - Int J Oncol. 2014 May;44(5):1561-70. doi: 10.3892/ijo.2014.2320. Epub 2014 Mar 5.