PMID- 24605332
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - rhPDGF-BB promotes proliferation and osteogenic differentiation of bone marrow 
      stromal cells from streptozotocin-induced diabetic rats through ERK pathway.
PG  - 637415
LID - 10.1155/2014/637415 [doi]
LID - 637415
AB  - Management of nonunion fracture and massive segmental bone defects in diabetes 
      remains a challenging clinical problem. Bone marrow stromal cells (BMSCs) are 
      crucial for bone remodeling and hold promise for bone regeneration. However, we 
      have showed previously that diabetes can affect the proliferation and osteogenic 
      potential of BMSCs adversely and a strategy to attenuate the impaired functions 
      of BMSCs is required. Platelet-derived growth factor-BB (PDGF-BB) plays an 
      important role in bone formation. However, little information is available about 
      its effect on diabetic BMSCs. In this study, BMSCs were isolated from 
      streptozotocin-induced diabetic rats. After treatment with recombinant human 
      PDGF-BB (rhPDGF-BB), diabetic BMSCs demonstrated enhanced cell proliferation and 
      osteogenic differentiation based on increased expressions of osteogenic genes 
      (Runx2, alkaline phosphatase, and osteocalcin) and Runx2 protein, as well as 
      upregulated alkaline phosphatase activity and mineralization. Furthermore, 
      blocking extracellular signal regulated kinase (ERK) pathway by inhibitor PD98059 
      repressed the enhanced proliferation and osteogenic differentiation in diabetic 
      BMSCs induced by rhPDGF-BB. Together, these results indicated that rhPDGF-BB 
      stimulates proliferation and osteogenic differentiation partially through ERK 
      pathway in diabetic BMSCs. Therefore, modulation of diabetic BMSCs could augment 
      BMSCs function affected by diabetes and holds significance for future strategies 
      to treat diabetic bone complications.
FAU - Zhao, Yanfang
AU  - Zhao Y
AD  - Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China ; 
      Department of Stomatology, Shanghai East Hospital Affiliated to Tongji 
      University, Shanghai 200011, China ; Shanghai Stomatological Diseases Center, 
      Shanghai 200001, China.
FAU - Zhang, Songmei
AU  - Zhang S
AD  - Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
FAU - Zeng, Deliang
AU  - Zeng D
AD  - Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
FAU - Xia, Lunguo
AU  - Xia L
AUID- ORCID: 0000-0001-6154-9652
AD  - Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated 
      to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
FAU - Lamichhane, Ashwini
AU  - Lamichhane A
AD  - Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
FAU - Jiang, Xinquan
AU  - Jiang X
AD  - Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China ; 
      Oral Bioengineering Laboratory/Regenerative Medicine Lab, Shanghai Key Laboratory 
      of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's 
      Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 639 
      Zhizaoju Road, Shanghai 200011, China.
FAU - Zhang, Fuqiang
AU  - Zhang F
AUID- ORCID: 0000-0003-2570-6248
AD  - Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140129
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Flavonoids)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 1B56C968OA (Becaplermin)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Becaplermin
MH  - Bone Marrow Cells/drug effects
MH  - Bone Regeneration/genetics
MH  - Cell Differentiation/*genetics
MH  - Cell Proliferation/drug effects
MH  - Diabetes Mellitus, Experimental/*genetics/pathology
MH  - Flavonoids/administration & dosage
MH  - Humans
MH  - MAP Kinase Signaling System/genetics
MH  - Osteogenesis/*genetics
MH  - Proto-Oncogene Proteins c-sis/*administration & dosage/metabolism
MH  - Rats
PMC - PMC3925525
EDAT- 2014/03/08 06:00
MHDA- 2014/10/16 06:00
PMCR- 2014/01/29
CRDT- 2014/03/08 06:00
PHST- 2013/04/30 00:00 [received]
PHST- 2013/09/30 00:00 [revised]
PHST- 2013/11/27 00:00 [accepted]
PHST- 2014/03/08 06:00 [entrez]
PHST- 2014/03/08 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
PHST- 2014/01/29 00:00 [pmc-release]
AID - 10.1155/2014/637415 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:637415. doi: 10.1155/2014/637415. Epub 2014 Jan 29.