PMID- 24605339
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - Modulation of c-Fos and BDNF protein expression in pentylenetetrazole-kindled 
      mice following the treatment with novel antiepileptic compound HHL-6.
PG  - 876712
LID - 10.1155/2014/876712 [doi]
LID - 876712
AB  - Brain-derived neurotrophic factor (BDNF) and c-Fos are shown to promote 
      epileptogenesis and are taken as a marker of neuronal activity. The present study 
      investigated the expression of BDNF and c-Fos in mice brain with 
      pentylenetetrazol- (PTZ-) induced generalized seizure and evaluated the effect of 
      novel tryptamine derivative HHL-6 on the expression of these two markers. The 
      subconvulsive dose of PTZ (50 mg/kg) was administered on alternate days in the 
      experimental groups until the seizure scores 4-5 developed in the PTZ-control 
      group. At the end of each experiment, animals were sacrificed, brain samples were 
      collected and cryosectioned, and immunohistochemical analysis of BDNF and c-Fos 
      protein was performed. Data obtained from two sections per mouse (n = 12 
      animals/group) is presented as means +/- S.E.M. The test compound HHL-6 
      demonstrated a potent anticonvulsant activity in the PTZ-induced seizure in mice. 
      Significant reduction in the BDNF (P < 0.003) and c-Fos (P < 0.01) protein 
      expression was observed in the HHL-6 treated group. Based on these results we 
      suggest that one of the possible mechanisms of HHL-6 to inhibit epileptogenesis 
      might be due to its controlling effect on the cellular and molecular expression 
      of the factors that contribute to the development of epileptogenic plasticity in 
      the CNS.
FAU - Malhi, Saima Mahmood
AU  - Malhi SM
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
FAU - Jawed, Huma
AU  - Jawed H
AUID- ORCID: 0000-0003-3643-4225
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
FAU - Hanif, Farina
AU  - Hanif F
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi 
      75270, Pakistan.
FAU - Ashraf, Nadeem
AU  - Ashraf N
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
FAU - Zubair, Farhat
AU  - Zubair F
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
FAU - Siddiqui, Bina S
AU  - Siddiqui BS
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
FAU - Begum, Sabira
AU  - Begum S
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
FAU - Kabir, Nurul
AU  - Kabir N
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi 
      75270, Pakistan.
FAU - Simjee, Shabana Usman
AU  - Simjee SU
AD  - H.E.J. Research Institute of Chemistry, International Center for Chemical and 
      Biological Sciences, University of Karachi, Karachi 75270, Pakistan ; Dr. 
      Panjwani Center for Molecular Medicine and Drug Research, International Center 
      for Chemical and Biological Sciences, University of Karachi, Karachi 75270, 
      Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20140129
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Anticonvulsants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Anticonvulsants/administration & dosage
MH  - Brain/drug effects/metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Disease Models, Animal
MH  - Epilepsy/*drug therapy/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Genes, fos/*genetics
MH  - Mice
MH  - Pentylenetetrazole/*administration & dosage
MH  - Seizures/*drug therapy/pathology
PMC - PMC3925558
EDAT- 2014/03/08 06:00
MHDA- 2014/10/16 06:00
PMCR- 2014/01/29
CRDT- 2014/03/08 06:00
PHST- 2013/04/15 00:00 [received]
PHST- 2013/10/22 00:00 [revised]
PHST- 2013/10/30 00:00 [accepted]
PHST- 2014/03/08 06:00 [entrez]
PHST- 2014/03/08 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
PHST- 2014/01/29 00:00 [pmc-release]
AID - 10.1155/2014/876712 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:876712. doi: 10.1155/2014/876712. Epub 2014 Jan 29.