PMID- 24607330
OWN - NLM
STAT- MEDLINE
DCOM- 20150115
LR  - 20220317
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 433
DP  - 2014 Jun 10
TI  - Improvement of specific detection of circulating tumor cells using combined CD45 
      staining and fluorescence in situ hybridization.
PG  - 69-75
LID - S0009-8981(14)00095-3 [pii]
LID - 10.1016/j.cca.2014.02.019 [doi]
AB  - BACKGROUND: Conventional cytokeratin (CK)-based methods to detect circulating 
      tumor cells (CTCs) often present suboptimal sensitivities for decreased or 
      non-expression of cytokeratin in some CTCs. We clinically investigated a new 
      method that combines immunocytochemistry staining (ICC) of CD45 and fluorescence 
      in situ hybridization (FISH). METHODS: Circulating epithelial cells from 141 
      subjects were enriched using an EpCAM-independent strategy and then identified by 
      either a combination of FISH with chromosome 8 centromere probe (CEP8) and ICC 
      staining of CD45 (CD45-FISH) or ICC staining of CK. RESULTS: For detecting CTCs 
      enriched from lung cancers, CD45-FISH had larger areas under ROC curves of 0.963 
      (P=0.000) compared to ICC (0.653; P=0.031) using cut-off values of 2 and 1 
      cell/3.75ml blood with sensitivities of 83.3% and 43.3%, specificities of 98.6% 
      and 89.5%, respectively. Moreover, CD45-FISH showed 76.2% sensitivity in 
      detecting CTCs in ovarian cancers (P<0.001). Four of six ovarian cancers showed 
      dramatical decrease in both CTCs and serum CA125 on the 7th day after surgery. 
      CONCLUSION: CD45-FISH method had improved sensitivity and specificity in 
      detecting CTCs of lung and ovarian cancers compared to ICC-CK. This combined 
      detection strategy may be useful in detecting or monitoring CTCs after ovarian 
      cancer surgery.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Ning, Ning
AU  - Ning N
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China; 
      Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin 
      University, Harbin, China.
FAU - Zhan, Ting
AU  - Zhan T
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Zhang, Yujuan
AU  - Zhang Y
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Chen, Qian
AU  - Chen Q
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Feng, Fengzhi
AU  - Feng F
AD  - Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, 
      Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      China.
FAU - Yang, Zhuo
AU  - Yang Z
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Liu, Zhongjuan
AU  - Liu Z
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Xu, Danfei
AU  - Xu D
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Wang, Fei
AU  - Wang F
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Guo, Ye
AU  - Guo Y
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Xing, Jia
AU  - Xing J
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Guan, Yuanyuan
AU  - Guan Y
AD  - Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, 
      Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      China.
FAU - Cui, Wei
AU  - Cui W
AD  - Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing, China. 
      Electronic address: wendycuiwei@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140304
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Cell Separation/*methods
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukocyte Common Antigens/*metabolism
MH  - Lung Neoplasms/pathology
MH  - Neoplastic Cells, Circulating/metabolism/*pathology
MH  - Ovarian Neoplasms/pathology
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - CD45-FISH
OT  - CTCs
OT  - ICC
OT  - Lung cancer
OT  - Ovarian tumor
EDAT- 2014/03/13 06:00
MHDA- 2015/01/16 06:00
CRDT- 2014/03/11 06:00
PHST- 2014/01/17 00:00 [received]
PHST- 2014/02/18 00:00 [accepted]
PHST- 2014/03/11 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2015/01/16 06:00 [medline]
AID - S0009-8981(14)00095-3 [pii]
AID - 10.1016/j.cca.2014.02.019 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2014 Jun 10;433:69-75. doi: 10.1016/j.cca.2014.02.019. Epub 2014 
      Mar 4.