PMID- 24607696 OWN - NLM STAT- MEDLINE DCOM- 20141112 LR - 20140317 IS - 1873-376X (Electronic) IS - 1570-0232 (Linking) VI - 953-954 DP - 2014 Mar 15 TI - Determination of bicuculline in rat plasma by liquid chromatography mass spectrometry and its application in a pharmacokinetic study. PG - 143-6 LID - S1570-0232(14)00088-9 [pii] LID - 10.1016/j.jchromb.2014.02.006 [doi] AB - Bicuculline, a phthalide isoquinoline alkaloid is of current interest as an antagonist of gamma-aminobutyric acid (GABA). A simple and sensitive liquid chromatography mass spectrometry method for determination of bicuculline in rat plasma was developed over the range of 5-500ng/mL. After addition of midazolam as internal standard, protein precipitation with acetonitrile-methanol (9:1, v/v) was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1mmx150mm, 5mum) column with acetonitrile -0.1% formic acid in water as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used for quantification using target fragment ions m/z 368 for bicuculline and m/z 326 for the IS. Linear calibration was obtained with correlation coefficients r>0.99. The CV of the precision measurements was less than 13%. The accuracy of the method ranged from 93.6% to 100.5%. Mean recoveries of bicuculline in plasma were in the range of 80.5-91.8%. The method was successfully applied to the pharmacokinetic study after gavage administration of 15mg/kg bicuculline in rats. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Ma, Jianshe AU - Ma J AD - Laboratory Animal Centre of Wenzhou Medical University, Wenzhou 325035, China. FAU - Lin, Chongliang AU - Lin C AD - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. FAU - Wen, Congcong AU - Wen C AD - Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China. FAU - Xiang, Zheng AU - Xiang Z AD - Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China. FAU - Yang, Xuezhi AU - Yang X AD - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. FAU - Wang, Xianqin AU - Wang X AD - Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China. Electronic address: lankywang@163.com. LA - eng PT - Journal Article DEP - 20140218 PL - Netherlands TA - J Chromatogr B Analyt Technol Biomed Life Sci JT - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JID - 101139554 RN - Y37615DVKC (Bicuculline) SB - IM MH - Animals MH - Bicuculline/*blood/chemistry/pharmacokinetics MH - Chromatography, Liquid/*methods MH - Drug Stability MH - Limit of Detection MH - Linear Models MH - Male MH - Mass Spectrometry/*methods MH - Rats MH - Rats, Sprague-Dawley MH - Reproducibility of Results OTO - NOTNLM OT - Bicuculline OT - LC-MS OT - Pharmacokinetics OT - Rat plasma EDAT- 2014/03/13 06:00 MHDA- 2014/11/13 06:00 CRDT- 2014/03/11 06:00 PHST- 2013/11/15 00:00 [received] PHST- 2014/01/16 00:00 [revised] PHST- 2014/02/10 00:00 [accepted] PHST- 2014/03/11 06:00 [entrez] PHST- 2014/03/13 06:00 [pubmed] PHST- 2014/11/13 06:00 [medline] AID - S1570-0232(14)00088-9 [pii] AID - 10.1016/j.jchromb.2014.02.006 [doi] PST - ppublish SO - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Mar 15;953-954:143-6. doi: 10.1016/j.jchromb.2014.02.006. Epub 2014 Feb 18.