PMID- 24607724
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20161125
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 55
DP  - 2014 May
TI  - Ghrelin receptor agonist, GHRP-2, produces antinociceptive effects at the 
      supraspinal level via the opioid receptor in mice.
PG  - 103-9
LID - S0196-9781(14)00057-6 [pii]
LID - 10.1016/j.peptides.2014.02.013 [doi]
AB  - GHRP-2 is a synthetic agonist of ghrelin receptor. GHRP-2 has similar 
      physiological functions with ghrelin. In our previous study, ghrelin (i.c.v.) 
      could induce analgesic effect through an interaction with GHS-R1alpha and with the 
      central opioid system in the acute pain in mice. To date, the function of GHRP-2 
      in pain processing was not understood. Therefore the aim of this study was to 
      investigate the effects of GHRP-2 on pain modulation at supraspinal level in mice 
      using the tail immersion test. Intracerebroventricular (i.c.v.) administration of 
      GHRP-2 (0.1, 0.3, 1, 3 and 10 nmol/L) produced a concentration- and time-related 
      antinociceptive effect. This effect could be fully antagonized by GHS-R1alpha 
      antagonist [d-Lys(3)]-GHRP-6, indicating that the analgesic effect induced by 
      GHRP-2 is mediated through the activation of GHS-R1alpha. Interestingly, naloxone, 
      naltrindole and nor-binaltorphimine, but not beta-funaltrexamine, could also block 
      the analgesic effect markedly, suggesting that delta- and kappa-opioid receptor is 
      involved in the analgesic response evoked by GHRP-2. Moreover, i.c.v. 
      administration of GHRP-2 potentiated the analgesic effect induced by morphine 
      (i.c.v., 1 nmol/L) and this potentiated effect could not be reversed by 
      [d-Lys(3)]-GHRP-6. Thus these findings may be a new strategy on investigating the 
      interaction between ghrelin system and opioids on pain modulation. Furthermore, 
      GHRP-2 may be a promising peptide for developing new analgesic drugs.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Zeng, Ping
AU  - Zeng P
AD  - Department of Physiology, Medical College of Nanchang University, Bayi Road 461, 
      Nanchang, Jiangxi 330006, China.
FAU - Li, Shu
AU  - Li S
AD  - Key Laboratory of Poyang Lake Environmental and Resource Utilization, Ministry of 
      Education, School of Environmental and Chemical Engineering, Nanchang University, 
      Nanchang, Jiangxi 330029, China.
FAU - Zheng, Yue-hui
AU  - Zheng YH
AD  - Medical Experimental Teaching Department, Nanchang University, Nanchang 330031, 
      China.
FAU - Liu, Fu-Yan
AU  - Liu FY
AD  - Department of Physiology, Medical College of Nanchang University, Bayi Road 461, 
      Nanchang, Jiangxi 330006, China.
FAU - Wang, Jing-lei
AU  - Wang JL
AD  - Department of Physiology, Medical College of Nanchang University, Bayi Road 461, 
      Nanchang, Jiangxi 330006, China.
FAU - Zhang, Da-lei
AU  - Zhang DL
AD  - Department of Physiology, Medical College of Nanchang University, Bayi Road 461, 
      Nanchang, Jiangxi 330006, China.
FAU - Wei, Jie
AU  - Wei J
AD  - Department of Physiology, Medical College of Nanchang University, Bayi Road 461, 
      Nanchang, Jiangxi 330006, China. Electronic address: jwei@ncu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140304
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Analgesics)
RN  - 0 (Analgesics, Opioid)
RN  - 0 (GHRP-6, Lys(3)-)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, Ghrelin)
RN  - 0 (Receptors, Opioid, delta)
RN  - 0 (Receptors, Opioid, kappa)
RN  - 36B82AMQ7N (Naloxone)
RN  - 76I7G6D29C (Morphine)
RN  - E6S6E1F19M (growth hormone-releasing peptide-2)
SB  - IM
MH  - Analgesics/*administration & dosage
MH  - Analgesics, Opioid/administration & dosage
MH  - Animals
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Morphine/administration & dosage
MH  - Naloxone/pharmacology
MH  - Narcotic Antagonists/pharmacology
MH  - Oligopeptides/*administration & dosage/pharmacology
MH  - Pain Perception/drug effects
MH  - Receptors, Ghrelin/antagonists & inhibitors
MH  - Receptors, Opioid, delta/*metabolism
MH  - Receptors, Opioid, kappa/*metabolism
OTO - NOTNLM
OT  - Antinociception
OT  - GHRP-2
OT  - Growth hormone secretagogue receptor 1 alpha (GHS-R1a)
OT  - Morphine
OT  - Opioid receptor
OT  - Tail withdrawal test
EDAT- 2014/03/13 06:00
MHDA- 2014/12/19 06:00
CRDT- 2014/03/11 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/02/20 00:00 [revised]
PHST- 2014/02/20 00:00 [accepted]
PHST- 2014/03/11 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
AID - S0196-9781(14)00057-6 [pii]
AID - 10.1016/j.peptides.2014.02.013 [doi]
PST - ppublish
SO  - Peptides. 2014 May;55:103-9. doi: 10.1016/j.peptides.2014.02.013. Epub 2014 Mar 
      4.