PMID- 24608385
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 39
IP  - 2
DP  - 2014 Feb
TI  - [Screening of differentially expressed genes and gene pathways in hepatitis C 
      virus 1b type nonstructural protein 4B stably expressed L02 cell line].
PG  - 117-23
LID - 10.11817/j.issn.1672-7347.2014.02.002 [doi]
AB  - OBJECTIVE: To screen differentially expressed genes and gene pathways in L02 cell 
      line stably expressing hepatitis C virus (HCV) Ib type nonstructural protein 4B 
      (NS4B) mediated by lentiviral system, and to provide a basis for further research 
      of molecular biological mechanism of NS4B gene in chronic hepatitis C and 
      hepatocarcinogenesis. METHODS: NS4B stably overexpressed L02 cell line and 
      negative control stable L02 cell line, designated as L02-NS4B and L02-mkate2 
      respectively, were resurrected and amplified in vitro. The differentially 
      expressed genes between L02-NS4B and L02-mkate2 were determined by gene 
      expression microarray from Human Gene 1.0ST. The significant pathways of the 
      differential genes were selected by the Fisher's exact test and chi2 test according 
      to kyoto encyclopedia of genes and genomes (KEGG) database. The differential 
      expression levels of 5 selected genes including protein kinase C delta binding 
      protein (PRKCDBP), tumor protein p53 (TP53), v-akt murine thymoma viral oncogene 
      homolog 1 (AKT1), baculoviral IAP repeat containing 3 (BIRC3) and B-cell lymphoma 
      2-like1 (BCL2L1) from cDNA microarray data were further verified by real-time 
      quantitative polymerase chain reaction (real-time QPCR). RESULTS: Between 
      L02-NS4B and L02-mkate2, the genes with flurescence intensity ratio >1.2 or <0.8 
      were considered as differentially expressed genes. A total of 2 682 
      differentially expressed genes in the known 28 869 human genes were detected in 
      L02-NS4B, 1 446 genes were upregulated and 1 236 genes were downregulated. A 
      total of 41 involved pathways of up-regulated differential genes were identified 
      by KEGG database, mainly including apoptosis, extracellular matrix receptor 
      interaction, cell cycle, pathways in cancer and Toll-like receptor signaling 
      pathway; and 20 involved pathways of down-regulated differential genes were 
      identified, mainly including pathways in cancer, Wnt signaling pathway and cell 
      cycle pathway. Of the 5 upregulated genes selected from cDNA microarray data, 3 
      genes showed the same differential expression pattern by real-time QPCR as that 
      shown in cDNA microarray data, namely AKT1, BIRC3 and BCL2L1. The confirmation 
      rate of real-time QPCR was 60%. CONCLUSION: HCVNS4B can up-regulate or 
      down-regulate the expression of many genes in L02 cells, thus affecting multiple 
      signaling pathways relevant to cell apoptosis, cell cycle and carcinogenesis.
FAU - Jiang, Xiaohua
AU  - Jiang X
AD  - Department of Infectious Diseases, Xiangya Hospital of Central South University, 
      Changsha 410087; Department of Infectious Diseases, the First Affiliated Hospital 
      of University of South China, Hengyang Hunan 421001,China.
FAU - Xie, Yutao
AU  - Xie Y
FAU - Cai, Yaping
AU  - Cai Y
FAU - Lei, Chuang
AU  - Lei C
FAU - Jiang, Bo
AU  - Jiang B
FAU - Peng, Hua
AU  - Peng H
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Cell Cycle
MH  - Cell Line
MH  - *Gene Expression Profiling
MH  - Hepacivirus/*genetics
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis
MH  - Real-Time Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Transcriptional Activation
MH  - Viral Nonstructural Proteins/*genetics
EDAT- 2014/03/13 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/03/11 06:00
PHST- 2014/03/11 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2014.02.002 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 Feb;39(2):117-23. doi: 
      10.11817/j.issn.1672-7347.2014.02.002.