PMID- 24609977
OWN - NLM
STAT- MEDLINE
DCOM- 20150126
LR  - 20240213
IS  - 1557-1904 (Electronic)
IS  - 1557-1890 (Print)
IS  - 1557-1890 (Linking)
VI  - 9
IP  - 3
DP  - 2014 Jun
TI  - Roles of glutathione in antioxidant defense, inflammation, and neuron 
      differentiation in the thalamus of HIV-1 transgenic rats.
PG  - 413-23
LID - 10.1007/s11481-014-9538-0 [doi]
AB  - Inflammation and oxidative stress in the brain are major causes of HIV-associated 
      neurocognitive disorders. Previously we have reported high content of glutathione 
      (GSH) in the thalamus of rats with F344 genetic background. In this study, we 
      investigated the changes of GSH metabolism and GSH-dependent antioxidant enzymes 
      in the rat thalamus in response to HIV-1 transgenesis, and their associations 
      with oxidative stress, inflammation, and neuronal development. Male HIV-1 
      transgenic (HIV-1Tg) rats and wild type F344 rats at 10 months were used in this 
      study, with 5 rats in each group. Parameters measured in this study included: 
      total and oxidized GSH, glutathione peroxidase (GPx), glutathione-S-transferase 
      (GST), gamma-glutamylcysteine synthetase (GCS), gamma-glutamyl transferase (GGT), 
      cysteine/cystine transporters, 4-hydroxynonenal (HNE), interleukin 12 (IL12), 
      neuronal nuclei (NeuN), microtubule-associated protein (MAP2), and glia 
      fibrillary acidic protein (GFAP). The levels of total GSH, oxidized GSH (GSSG) 
      and MAP2 protein, and enzymatic activities of GCS, GPx and GST were significantly 
      higher in HIV-1Tg rats compared with F344 rats, but the ratio of GSSG/GSH, 
      activity of GGT and levels of HNE, NeuN protein and GFAP protein did not change. 
      HIV-1Tg rats showed a lower level of IL12 protein. GSH positively correlated with 
      GCS, GST and MAP2, GSSG/GSH ratio positively correlated with HNE and IL12, the 
      activities of GPx, GST and GCS positively correlated with each other, and 
      negatively correlated with HNE. These findings suggest an important role of the 
      GSH-centered system in reducing oxidative stress and neuroinflammation, and 
      enhancing neuron differentiation in the thalamus of HIV-1Tg rats.
FAU - Pang, Xiaosha
AU  - Pang X
AD  - Department of Cell and Molecular Biology, John A. Burns School of Medicine, 
      University of Hawaii at Manoa, 651 Ilalo Street BSB 222, Honolulu, HI, 96813, 
      USA.
FAU - Panee, Jun
AU  - Panee J
LA  - eng
GR  - G12 MD007601/MD/NIMHD NIH HHS/United States
GR  - R21 AT003874/AT/NCCIH NIH HHS/United States
GR  - 5G12RR003061/RR/NCRR NIH HHS/United States
GR  - U54 MD007584/MD/NIMHD NIH HHS/United States
GR  - P20 RR016467/RR/NCRR NIH HHS/United States
GR  - G12 RR003061/RR/NCRR NIH HHS/United States
GR  - 8G12MD007601/MD/NIMHD NIH HHS/United States
GR  - 5P20RR016467-11/RR/NCRR NIH HHS/United States
GR  - R21 AT005139/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140308
PL  - United States
TA  - J Neuroimmune Pharmacol
JT  - Journal of neuroimmune pharmacology : the official journal of the Society on 
      NeuroImmune Pharmacology
JID - 101256586
RN  - 0 (Antioxidants)
RN  - 0 (Inflammation Mediators)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Antioxidants/*physiology
MH  - Cell Differentiation/*physiology
MH  - Glutathione/*physiology
MH  - HIV-1/*physiology
MH  - Inflammation/metabolism
MH  - Inflammation Mediators/*physiology
MH  - Male
MH  - Neurons/physiology
MH  - Oxidative Stress/physiology
MH  - Rats
MH  - Rats, Transgenic
MH  - Thalamus/*physiology
PMC - PMC4868348
MID - NIHMS784258
EDAT- 2014/03/13 06:00
MHDA- 2015/01/27 06:00
PMCR- 2016/05/16
CRDT- 2014/03/11 06:00
PHST- 2013/10/14 00:00 [received]
PHST- 2014/02/23 00:00 [accepted]
PHST- 2014/03/11 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2015/01/27 06:00 [medline]
PHST- 2016/05/16 00:00 [pmc-release]
AID - 10.1007/s11481-014-9538-0 [doi]
PST - ppublish
SO  - J Neuroimmune Pharmacol. 2014 Jun;9(3):413-23. doi: 10.1007/s11481-014-9538-0. 
      Epub 2014 Mar 8.