PMID- 24611149
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141216
LR  - 20240321
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 16
IP  - 3
DP  - 2014 Fall
TI  - Comparative study on functional effects of allotransplantation of bone marrow 
      stromal cells and adipose derived stromal vascular fraction on tendon repair: a 
      biomechanical study in rabbits.
PG  - 263-70
AB  - OBJECTIVE: Tendon never returns to its complete biological and mechanical 
      properties after repair. Bone marrow and, recently, adipose tissue have been used 
      as sources of mesenchymal stem cells which have been proven to enhance tendon 
      healing. In the present study, we compared the effects of allotransplantation of 
      bone marrow derived mesenchymal stromal cells (BMSCs) and adipose derived stromal 
      vascular fraction (SVF) on tendon mechanical properties after experimentally 
      induced flexor tendon transection. MATERIALS AND METHODS: In this experimental 
      study, we used 48 adult male New Zealand white rabbits. Twelve of rabbits were 
      used as donors of bone marrow and adipose tissue, the rest were divided into 
      control and treatment groups. The injury model was a unilateral complete 
      transection of the deep digital flexor tendon. Immediately after suture repair, 
      4x10(6)cells of either fresh SVF from enzymatic digestion of adipose tissue or 
      cultured BMSCs were intratendinously injected into tendon stumps in the treatment 
      groups. Controls received phosphate-buffered saline (PBS). Immobilization with a 
      cast was continued for two weeks after surgery. Animals were sacrificed three and 
      eight weeks after surgery and tendons underwent mechanical evaluations. The 
      differences among the groups were analyzed using the analysis of variance (ANOVA) 
      test followed by Tukey's multiple comparisons test. RESULTS: Stromal cell 
      transplantation resulted in a significant increase in ultimate and yield loads, 
      energy absorption, and stress of repairs compared to the controls. However, there 
      were no statistically significant changes detected in terms of stiffness. In 
      comparison, we observed no significant differences at the third week between SVF 
      and BMSCs treated tendons in terms of all load related properties. However, at 
      the eighth week SVF transplantation resulted in significantly increased energy 
      absorption, stress and stiffness compared to BMSCs. CONCLUSION: The enhanced 
      biomechanical properties of repairs in this study advocates the application of 
      adipose derived SVF as an excellent source of multipotent cells instead of 
      traditional BMSCs and may seem more encouraging in cell-based therapy for tendon 
      injuries.
FAU - Behfar, Mehdi
AU  - Behfar M
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia 
      University, Urmia, Iran.
FAU - Javanmardi, Sara
AU  - Javanmardi S
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of 
      Tabriz, Tabriz, Iran.
FAU - Sarrafzadeh-Rezaei, Farshid
AU  - Sarrafzadeh-Rezaei F
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia 
      University, Urmia, Iran.
LA  - eng
PT  - Journal Article
DEP - 20141004
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC4204193
OTO - NOTNLM
OT  - Adipose Tissue
OT  - Bone Marrow
OT  - Tendon
OT  - Tensile Strength
OT  - Transplantation
EDAT- 2014/03/13 06:00
MHDA- 2014/03/13 06:01
PMCR- 2014/09/01
CRDT- 2014/03/11 06:00
PHST- 2013/05/19 00:00 [received]
PHST- 2013/09/09 00:00 [accepted]
PHST- 2014/03/11 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/03/13 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - CellJ.2014.16(3).6 [pii]
PST - ppublish
SO  - Cell J. 2014 Fall;16(3):263-70. Epub 2014 Oct 4.