PMID- 24611833
OWN - NLM
STAT- MEDLINE
DCOM- 20140527
LR  - 20181202
IS  - 1365-3024 (Electronic)
IS  - 0141-9838 (Linking)
VI  - 36
IP  - 5
DP  - 2014 May
TI  - Dogs vaccinated with gentamicin-attenuated Leishmania infantum or infected with 
      wild-type parasite can be distinguished by Western blotting.
PG  - 218-24
LID - 10.1111/pim.12108 [doi]
AB  - An attenuated line of Leishmania infantum (the H-line), developed through 
      exposure to gentamicin, has been shown to protect dogs against canine visceral 
      leishmaniasis. A specific diagnostic test to differentiate dogs vaccinated with 
      the attenuated line from dogs infected with L. infantum wild-type (L. infantum 
      WT) could be a valuable tool in evaluating the effectiveness of canine 
      vaccination. In this study, 28 healthy dogs were allocated into four groups. In 
      Group I and Group II (eight dogs per group), dogs were immunized subcutaneously 
      (s.c.) with L. infantum H-line, and the dogs of Group II challenged s.c. with 
      L. infantum WT, at 2 months post-immunization. In Group III, eight animals were 
      challenged s.c. with L. infantum WT, and four dogs of Group IV were injected s.c. 
      with PBS. We found that sera from vaccinated dogs recognize a 21 kDa antigen of 
      promastigotes of L. infantum H-line but not of L. infantum WT, whereas sera from 
      unvaccinated dogs challenged with L. infantum WT, recognized a 21 kDa antigen of 
      promastigotes of L. infantum WT but not of L. infantum H-line. Sera from dogs 
      challenged with L. infantum WT with prior vaccination with L. infantum H-line, 
      recognized a 21 kDa antigen of both L. infantum WT and L. infantum H-line. These 
      results suggest that the Western blot analysis of antibodies against 21 kDa 
      antigens of L. infantum H-line and WT may be a useful technique for 
      distinguishing between dogs vaccinated with L. infantum H-line and dogs naturally 
      infected with L. infantum WT.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Daneshvar, H
AU  - Daneshvar H
AD  - Leishmaniasis Center, Kerman University of Medical Sciences, Kerman, Iran.
FAU - Mahmmodi, Z
AU  - Mahmmodi Z
FAU - Kamiabi, H
AU  - Kamiabi H
FAU - Phillips, R S
AU  - Phillips RS
FAU - Burchmore, R
AU  - Burchmore R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Gentamicins)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/*blood
MH  - Antigens, Protozoan/*immunology
MH  - Blotting, Western
MH  - Dog Diseases/*immunology/prevention & control
MH  - Dogs
MH  - Female
MH  - Gentamicins
MH  - Immunoglobulin G/blood
MH  - Leishmania infantum/*immunology
MH  - Leishmaniasis, Visceral/*immunology/prevention & control/veterinary
MH  - Male
MH  - Vaccination
OTO - NOTNLM
OT  - Western blot
OT  - distinguishing
OT  - dogs
OT  - gentamicin-attenuated Leishmania infantum
OT  - vaccine
EDAT- 2014/03/13 06:00
MHDA- 2014/05/28 06:00
CRDT- 2014/03/12 06:00
PHST- 2013/11/03 00:00 [received]
PHST- 2014/02/18 00:00 [accepted]
PHST- 2014/03/12 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/05/28 06:00 [medline]
AID - 10.1111/pim.12108 [doi]
PST - ppublish
SO  - Parasite Immunol. 2014 May;36(5):218-24. doi: 10.1111/pim.12108.