PMID- 24612951
OWN - NLM
STAT- MEDLINE
DCOM- 20140509
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 170
IP  - 1-2
DP  - 2014 May 14
TI  - Infection of differentiated airway epithelial cells from caprine lungs by viruses 
      of the bovine respiratory disease complex.
PG  - 58-64
LID - S0378-1135(14)00073-X [pii]
LID - 10.1016/j.vetmic.2014.01.038 [doi]
AB  - Bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus type 3 
      (BPIV3) and bovine herpesvirus type 1 (BHV-1) are important pathogens associated 
      with the bovine respiratory disease complex (BRDC). Non-bovine ruminants such as 
      goats may also be infected and serve as a virus reservoir to be considered in the 
      development of control strategies. To evaluate the susceptibility of caprine 
      airway epithelial cells to infection by viruses of BRDC, we established a culture 
      system for differentiated caprine epithelial cells. For this purpose, we 
      generated precision-cut lung slices (PCLS), in which cells are retained in their 
      original structural configuration and remain viable for more than a week. The 
      three bovine viruses were found to preferentially infect different cell types. 
      Ciliated epithelial cells were the major target cells of BPIV3, whereas BHV-1 
      preferred basal cells. Cells infected by BRSV were detected in submucosal cell 
      layers. This spectrum of susceptible cells is the same as that reported recently 
      for infected bovine PCLS. While infection of caprine cells by BRSV and BPIV3 was 
      as efficient as that reported for bovine cells, infection of caprine cells by 
      BHV-1 required a tenfold higher dose of infectious virus as compared to infection 
      of bovine airway cells. These results support the notion that non-bovine 
      ruminants may serve as a reservoir for viruses of BRDC and introduce a culture 
      system to analyze virus infection of differentiated airway epithelial cells from 
      the caprine lung.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kirchhoff, Jana
AU  - Kirchhoff J
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Uhlenbruck, Sabine
AU  - Uhlenbruck S
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Keil, Gunther M
AU  - Keil GM
AD  - Institute of Molecular Biology, Friedrich-Loeffler-Institut, Greifswald-Insel 
      Riems, Germany.
FAU - Schwegmann-Wessels, Christel
AU  - Schwegmann-Wessels C
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany.
FAU - Ganter, Martin
AU  - Ganter M
AD  - Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, 
      University of Veterinary Medicine, Hannover, Germany.
FAU - Herrler, Georg
AU  - Herrler G
AD  - Institute of Virology, University of Veterinary Medicine Hannover, Hannover, 
      Germany. Electronic address: georg.herrler@tiho-hannover.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140209
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Bovine Respiratory Disease Complex/*virology
MH  - Cattle
MH  - Cells, Cultured
MH  - Disease Reservoirs/*veterinary
MH  - Epithelial Cells/virology
MH  - Goat Diseases/*virology
MH  - Goats
MH  - Herpesvirus 1, Bovine/physiology
MH  - *Host-Pathogen Interactions
MH  - Parainfluenza Virus 3, Bovine/physiology
MH  - Respiratory Mucosa/cytology/*virology
MH  - Respiratory Syncytial Virus, Bovine/physiology
OTO - NOTNLM
OT  - Bovine herpesvirus type 1
OT  - Bovine parainfluenza virus type 3
OT  - Bovine respiratory disease complex
OT  - Bovine respiratory syncytial virus
OT  - Goat respiratory disease
OT  - Precision-cut lung slices
EDAT- 2014/03/13 06:00
MHDA- 2014/05/10 06:00
CRDT- 2014/03/12 06:00
PHST- 2013/12/12 00:00 [received]
PHST- 2014/01/27 00:00 [revised]
PHST- 2014/01/29 00:00 [accepted]
PHST- 2014/03/12 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/05/10 06:00 [medline]
AID - S0378-1135(14)00073-X [pii]
AID - 10.1016/j.vetmic.2014.01.038 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 May 14;170(1-2):58-64. doi: 10.1016/j.vetmic.2014.01.038. 
      Epub 2014 Feb 9.