PMID- 24613849
OWN - NLM
STAT- MEDLINE
DCOM- 20140522
LR  - 20140407
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 446
IP  - 2
DP  - 2014 Apr 4
TI  - Utility of fluorescence in situ hybridization for ploidy and p57 immunostaining 
      in discriminating hydatidiform moles.
PG  - 555-60
LID - S0006-291X(14)00418-5 [pii]
LID - 10.1016/j.bbrc.2014.03.003 [doi]
AB  - Discrimination between complete moles (CMs), partial moles (PMs), and hydropic 
      abortions (HAs) is important as the risk of persistent gestational trophoblastic 
      disease (GTD) differs for each condition. We evaluated whether ancillary 
      fluorescence in situ hybridization (FISH) with a set of chromosome enumeration 
      probes (CEP) for chromosomes X, Y, and 17 and p57 immunostaining could improve 
      the clinical diagnosis. Forty-one products of conception (POC) were reclassified 
      according to clinical performance, morphology, p57 immunostaining results, and 
      FISH results. The accuracy of histological examination alone was 85% for the 
      original diagnosis. FISH analysis showed diploidy in 19 of 20 CMs and triploidy 
      in 4 of 6 PMs. The concordance rate was 92.5% on using the CEP probes. p57 
      Staining was negative in all CMs and positive in all PMs and HAs. Chromosomal 
      abnormality was detected in 3 cases of HA by using FISH. In conclusion, combined 
      p57 immunostaining and FISH with a set of 3 CEP probes for chromosomes X, Y, and 
      17 could be useful in the classification of hydatidiform moles.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Chen, Kuang-Hua
AU  - Chen KH
AD  - Department of Pathology, Chang Gung Memorial Hospital, Chang Gung University 
      School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Hsu, Sheng-Chi
AU  - Hsu SC
AD  - Cancer Diagnostic Laboratory, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
FAU - Chen, Hou-Yu
AU  - Chen HY
AD  - Department of Pathology, Chang Gung Memorial Hospital, Chang Gung University 
      School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Ng, Kwai-Fong
AU  - Ng KF
AD  - Department of Pathology, Chang Gung Memorial Hospital, Chang Gung University 
      School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Chen, Tse-Ching
AU  - Chen TC
AD  - Department of Pathology, Chang Gung Memorial Hospital, Chang Gung University 
      School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan. Electronic address: 
      ctc323@cgmh.org.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140312
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CDKN1C protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p57)
SB  - IM
MH  - Adult
MH  - Cyclin-Dependent Kinase Inhibitor p57/*metabolism
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Hydatidiform Mole/genetics/*metabolism/*pathology
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Ploidies
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Pregnancy
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Uterine Neoplasms
MH  - Young Adult
OTO - NOTNLM
OT  - Chromosome enumeration probes
OT  - Fluorescence in situ hybridization
OT  - Histopathology
OT  - Hydatidiform mole
OT  - p57 Immunohistochemistry
EDAT- 2014/03/13 06:00
MHDA- 2014/05/23 06:00
CRDT- 2014/03/12 06:00
PHST- 2014/02/25 00:00 [received]
PHST- 2014/03/02 00:00 [accepted]
PHST- 2014/03/12 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/05/23 06:00 [medline]
AID - S0006-291X(14)00418-5 [pii]
AID - 10.1016/j.bbrc.2014.03.003 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Apr 4;446(2):555-60. doi: 
      10.1016/j.bbrc.2014.03.003. Epub 2014 Mar 12.