PMID- 24614617
OWN - NLM
STAT- MEDLINE
DCOM- 20160331
LR  - 20220316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 3
DP  - 2014
TI  - Boosting in planta production of antigens derived from the porcine reproductive 
      and respiratory syndrome virus (PRRSV) and subsequent evaluation of their 
      immunogenicity.
PG  - e91386
LID - 10.1371/journal.pone.0091386 [doi]
LID - e91386
AB  - Porcine reproductive and respiratory syndrome (PRRS) is a disease of swine, 
      caused by an arterivirus, the PRRS virus (PRRSV). This virus infects pigs 
      worldwide and causes huge economic losses. Due to genetic drift, current vaccines 
      are losing their power. Adaptable vaccines could provide a solution to this 
      problem. This study aims at producing in planta a set of antigens derived from 
      the PRRSV glycoproteins (GPs) to be included in a subunit vaccine. We selected 
      the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed 
      platform by removing transmembrane domains (Tm) and/or adding stabilizing protein 
      domains, such as the green fluorescent protein (GFP) and immunoglobulin (IgG) 
      'Fragment crystallizable' (Fc) chains. Accumulation of the GPs with and without 
      Tm was low, reaching no more than 0.10% of total soluble protein (TSP) in 
      homozygous seed. However, addition of stabilizing domains boosted accumulation up 
      to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also 
      the Fc chains of the porcine IgG3 and murine IgG2a increased antigen 
      accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc 
      chain did not. Antigens with Tm were less susceptible to these manipulations to 
      increase yield. All antigens were produced in the endoplasmic reticulum and 
      accordingly, they carried high-mannose N-glycans. The immunogenicity of several 
      of those antigens was assessed and we show that vaccination with purified 
      antigens did elicit the production of antibodies with virus neutralizing activity 
      in mice but not in pigs.
FAU - Piron, Robin
AU  - Piron R
AD  - Department of Plant Systems Biology, VIB, Ghent, Belgium; Department of Plant 
      Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
FAU - De Koker, Stefaan
AU  - De Koker S
AD  - Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
FAU - De Paepe, Annelies
AU  - De Paepe A
AD  - Department of Plant Systems Biology, VIB, Ghent, Belgium; Department of Plant 
      Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
FAU - Goossens, Julie
AU  - Goossens J
AD  - Department of Bioscience Engineering, VUB, Brussels, Belgium.
FAU - Grooten, Johan
AU  - Grooten J
AD  - Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
FAU - Nauwynck, Hans
AU  - Nauwynck H
AD  - Department of Virology, Parasitology and Immunology, Ghent University, Ghent, 
      Belgium.
FAU - Depicker, Ann
AU  - Depicker A
AD  - Department of Plant Systems Biology, VIB, Ghent, Belgium; Department of Plant 
      Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
LA  - eng
SI  - GENBANK/KF983786
SI  - GENBANK/KF983787
SI  - GENBANK/KF983788
SI  - GENBANK/KF983789
SI  - GENBANK/KF983790
SI  - GENBANK/KF983791
SI  - GENBANK/KF983792
SI  - GENBANK/KF983793
SI  - GENBANK/KF983794
SI  - GENBANK/KF983795
SI  - GENBANK/KF983796
SI  - GENBANK/KF983797
SI  - GENBANK/KF983798
SI  - GENBANK/KF983799
SI  - GENBANK/KF983800
SI  - GENBANK/KF983801
SI  - GENBANK/KF983802
SI  - GENBANK/KF983803
SI  - GENBANK/KF983804
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140310
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Glycoproteins)
RN  - 0 (Polysaccharides)
RN  - 0 (T-DNA)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/biosynthesis/*immunology/isolation & purification
MH  - Arabidopsis/genetics/*metabolism
MH  - Chromatography, Affinity
MH  - DNA, Bacterial/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Glycoproteins/*immunology
MH  - Glycosylation
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Molecular Sequence Data
MH  - Plants, Genetically Modified
MH  - Polysaccharides/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Seeds/metabolism
MH  - Swine/*virology
PMC - PMC3948849
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/03/13 06:00
MHDA- 2016/04/01 06:00
PMCR- 2014/03/10
CRDT- 2014/03/12 06:00
PHST- 2013/12/10 00:00 [received]
PHST- 2014/02/10 00:00 [accepted]
PHST- 2014/03/12 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2016/04/01 06:00 [medline]
PHST- 2014/03/10 00:00 [pmc-release]
AID - PONE-D-13-52045 [pii]
AID - 10.1371/journal.pone.0091386 [doi]
PST - epublish
SO  - PLoS One. 2014 Mar 10;9(3):e91386. doi: 10.1371/journal.pone.0091386. eCollection 
      2014.