PMID- 24615723
OWN - NLM
STAT- MEDLINE
DCOM- 20141010
LR  - 20140404
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 53
IP  - 6
DP  - 2014 Jun
TI  - Frequent translocations of 11q13.2 and 19p13.2 in ovarian cancer.
PG  - 447-53
LID - 10.1002/gcc.22152 [doi]
AB  - Aberrations of chromosome arm 19p in ovarian cancer were first described decades 
      ago and have been confirmed in recent publications, which have focused on 
      chromosome 11 as a translocation partner. Recently, genetic analysis of the 
      ovarian cancer cell line SKOV3 revealed a rearrangement described as 
      der(19)t(11;19)(q13.2;p13.2), which lead to a fusion protein containing parts of 
      HOOK2 and frame shifted ACTN3 that had unknown functionality. To evaluate the 
      frequency of these breakpoints, we used fluorescence in situ hybridization (FISH) 
      probes flanking these genes for interphase analysis of ovarian cancer cells. We 
      analyzed 49 primary cell cultures of ovarian cancers using FISH probes next to 
      these breakpoints on chromosomes 11 and 19 defined in SKOV3. Co-localizations of 
      the signals in interphase nuclei were considered to be positive fusions when the 
      frequency was over the experimentally calculated cutoff of 24.3% (mean average 
      value for normal ovary cells plus three times the standard deviation). Fusions 
      between 11q13.2 and 19p13.2 were confirmed in 22 (45%) primary cell cultures of 
      ovarian cancers. However, by PCR, the fusion originally described in SKOV3 was 
      not detected in any of the primary cell cultures. Our results confirm other 
      reports and show that these regions are very frequently involved in chromosomal 
      rearrangements in ovarian cancer. Furthermore, they reveal a significant 
      correlation (P = 0.023) of co-localized signals of 11q13.2 and 19p13.2 with low 
      and intermediate grades in ovarian cancer.
CI  - Copyright (c) 2014 Wiley Periodicals, Inc.
FAU - Wang, Liang
AU  - Wang L
AD  - Department of Gynecology, Zhejiang University School of Medicine, The 2nd 
      Affiliated Hospital, Hangzhou, Zhejiang, China; Clinic of Gynecology and 
      Obstetrics, University Hospital Schleswig-Holstein Campus Kiel, Kiel, 
      Schleswig-Holstein, Germany.
FAU - Wenners, Antonia
AU  - Wenners A
FAU - Hilpert, Felix
AU  - Hilpert F
FAU - Fredrik, Regina
AU  - Fredrik R
FAU - Micci, Francesca
AU  - Micci F
FAU - Onkes, Wiebke
AU  - Onkes W
FAU - Caliebe, Amke
AU  - Caliebe A
FAU - Maass, Nicolai
AU  - Maass N
FAU - Weimer, Jorg
AU  - Weimer J
FAU - Arnold, Norbert
AU  - Arnold N
LA  - eng
PT  - Journal Article
DEP - 20140224
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Cell Line, Tumor
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 19
MH  - Female
MH  - Gene Fusion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Ovarian Neoplasms/*genetics/pathology
MH  - *Translocation, Genetic
EDAT- 2014/03/13 06:00
MHDA- 2014/10/11 06:00
CRDT- 2014/03/12 06:00
PHST- 2013/08/23 00:00 [received]
PHST- 2014/01/17 00:00 [revised]
PHST- 2014/01/19 00:00 [accepted]
PHST- 2014/03/12 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/10/11 06:00 [medline]
AID - 10.1002/gcc.22152 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2014 Jun;53(6):447-53. doi: 10.1002/gcc.22152. Epub 
      2014 Feb 24.