PMID- 24618842
OWN - NLM
STAT- MEDLINE
DCOM- 20150529
LR  - 20220129
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 3
DP  - 2014
TI  - Global gene expression profiling of myeloid immune cell subsets in response to in 
      vitro challenge with porcine circovirus 2b.
PG  - e91081
LID - 10.1371/journal.pone.0091081 [doi]
LID - e91081
AB  - Compelling evidence suggests that the early interaction between porcine 
      circovirus 2 (PCV-2) and the innate immune system is the key event in the 
      pathogenesis of Post-Weaning Multisystemic Wasting Syndrome (PMWS). Furthermore, 
      PCV2 has been detected in bone-marrow samples, potentially enabling an easy 
      spread and reservoir for the virus. To assess the gene-expression differences 
      induced by an in-vitro PCV2b infection in different three different myeloid 
      innate immune cell subsets generated from the same animal, we used the Agilent 
      Porcine Gene Expression Microarray (V2). Alveolar macrophages (AMOs), 
      monocyte-derived dendritic cells (MoDCs) and bone-marrow cells (BMCs) were 
      generated from each animal, and challenged with a UK-isolate of a PCV2 genotype 
      b-strain at a MOI of 0.5. Remarkably, analysis showed a highly distinct and 
      cell-type dependent response to PCV2b challenge. Overall, MoDCs showed the most 
      marked response to PCV2b challenge in vitro and revealed a key role for TNF in 
      the interaction with PCV2b, whereas only few genes were affected in BMCs and 
      AMOs. These observations were further supported by an enrichment of genes in the 
      downstream NF-kappaB Signalling pathway as well as an up regulation of genes with 
      pro-apoptotic functions post-challenge. PCV2b challenge increases the expression 
      of a large number of immune-related and pro-apoptotic genes mainly in MoDC, which 
      possibly explain the increased inflammation, granulomatous inflammation and 
      lymphocyte depletion seen in PMWS-affected pigs.
FAU - Mavrommatis, Bettina
AU  - Mavrommatis B
AD  - The Royal Veterinary College, Hatfield, United Kingdom.
FAU - Offord, Victoria
AU  - Offord V
AD  - The Royal Veterinary College, Hatfield, United Kingdom.
FAU - Patterson, Robert
AU  - Patterson R
AD  - The Royal Veterinary College, Hatfield, United Kingdom.
FAU - Watson, Mick
AU  - Watson M
AD  - ARK-Genomics, The Roslin Institute & R(D)SVS, University of Edinburgh, 
      Midlothian, Edinburgh, United Kingdom.
FAU - Kanellos, Theo
AU  - Kanellos T
AD  - Zoetis Animal Health, Paris, France.
FAU - Steinbach, Falko
AU  - Steinbach F
AD  - Department of Virology, Animal Health and Veterinary Laboratories Agency, 
      Addlestone, United Kingdom.
FAU - Grierson, Sylvia
AU  - Grierson S
AD  - Department of Virology, Animal Health and Veterinary Laboratories Agency, 
      Addlestone, United Kingdom.
FAU - Werling, Dirk
AU  - Werling D
AD  - The Royal Veterinary College, Hatfield, United Kingdom.
LA  - eng
GR  - BB/E018394/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BBS/E/D/20211552/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BB/FO18394/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140311
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics/immunology
MH  - Bone Marrow Cells/immunology/metabolism/virology
MH  - Circoviridae Infections/*veterinary
MH  - Circovirus/*immunology
MH  - Dendritic Cells/immunology/metabolism/virology
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Macrophages, Alveolar/immunology/metabolism/virology
MH  - Molecular Sequence Annotation
MH  - Myeloid Cells/*immunology/*metabolism/virology
MH  - Signal Transduction
MH  - Swine
MH  - Swine Diseases/*genetics/*immunology/virology
MH  - Time Factors
MH  - Transcriptome
PMC - PMC3949749
COIS- Competing Interests: Theo Kanellos is an employee of Zoetis Animal Health, Paris, 
      whose company provided funding towards this study. Biobest Laboratories also 
      provided funding for this study. There are no patents, products in development or 
      marketed products to declare. This does not alter our adherence to all the PLOS 
      ONE policies on sharing data and materials.
EDAT- 2014/03/13 06:00
MHDA- 2015/05/30 06:00
PMCR- 2014/03/11
CRDT- 2014/03/13 06:00
PHST- 2013/10/28 00:00 [received]
PHST- 2014/02/07 00:00 [accepted]
PHST- 2014/03/13 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2015/05/30 06:00 [medline]
PHST- 2014/03/11 00:00 [pmc-release]
AID - PONE-D-13-44339 [pii]
AID - 10.1371/journal.pone.0091081 [doi]
PST - epublish
SO  - PLoS One. 2014 Mar 11;9(3):e91081. doi: 10.1371/journal.pone.0091081. eCollection 
      2014.