PMID- 2461965
OWN - NLM
STAT- MEDLINE
DCOM- 19890120
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 82
IP  - 6
DP  - 1988 Dec
TI  - Urokinase-dependent adhesion loss and shape change after cyclic adenosine 
      monophosphate elevation in cultured rat mesangial cells.
PG  - 1992-2000
AB  - Mesangial cells in culture change shape and become less adhesive in response to 
      cAMP elevation (e.g., treatment with isoproterenol plus isobutylmethylxanthine 
      (IM). Inhibitors of serine proteases inhibit cellular shape change in response to 
      IM. To further examine the role of cell surface proteases in shape change, 
      adhesion plaque proteins (i.e., preparations of ventral membranes and 
      extracellular matrix) were separated in SDS-polyacrylamide gels containing 
      gelatin with and without plasminogen. Four discrete zones of lysis were evident 
      in plasminogen gels (indicative of activation of plasminogen) from control 
      adhesion plaques: one inconspicuous zone with a Mr approximately 150 kD, another 
      at approximately 115 kD, and a doublet at approximately 35-32 kD. Another diffuse 
      zone of lysis centered around Mr approximately 70 kD and contained a defined band 
      of approximately 56 kD. Adhesion plaques contained most of the plasminogen 
      activators (PA). 5 min after IM treatment, the Mr approximately 150- and 
      approximately 115-kD PA were increased in activity. Vasopressin (VP), which 
      prevented shape change and adhesion loss when added along with IM, inhibited the 
      increase in these PA. Preincubation with monoclonal or polyclonal antibodies to 
      urokinase-type plasminogen activator (uPA) totally inhibited the IM-inducible 
      shape change and adhesion loss. Activation of plasminogen throughout the gels 
      revealed multiple protease resistant bands that markedly increased with IM 
      treatment (maximal at 45 min). These may represent focal control mechanisms. uPA 
      thus may mediate focal proteolysis, which results in shape change and decreased 
      adhesion.
FAU - Glass, W F 2nd
AU  - Glass WF 2nd
AD  - Department of Pathology, University of Texas, San Antonio 78284.
FAU - Radnik, R A
AU  - Radnik RA
FAU - Garoni, J A
AU  - Garoni JA
FAU - Kreisberg, J I
AU  - Kreisberg JI
LA  - eng
GR  - DK-17387/DK/NIDDK NIH HHS/United States
GR  - DK-29787/DK/NIDDK NIH HHS/United States
GR  - DK-37139/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - L628TT009W (Isoproterenol)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Animals
MH  - *Cell Adhesion
MH  - Cells, Cultured
MH  - Cyclic AMP/*metabolism
MH  - Glomerular Mesangium/*metabolism
MH  - Isoproterenol/pharmacology
MH  - Male
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Urokinase-Type Plasminogen Activator/*metabolism
PMC - PMC442781
EDAT- 1988/12/01 00:00
MHDA- 1988/12/01 00:01
PMCR- 1988/12/01
CRDT- 1988/12/01 00:00
PHST- 1988/12/01 00:00 [pubmed]
PHST- 1988/12/01 00:01 [medline]
PHST- 1988/12/01 00:00 [entrez]
PHST- 1988/12/01 00:00 [pmc-release]
AID - 10.1172/JCI113819 [doi]
PST - ppublish
SO  - J Clin Invest. 1988 Dec;82(6):1992-2000. doi: 10.1172/JCI113819.