PMID- 24619669
OWN - NLM
STAT- MEDLINE
DCOM- 20141114
LR  - 20140312
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1139
DP  - 2014
TI  - Ex vivo loading of autologous dendritic cells with tumor antigens.
PG  - 41-4
LID - 10.1007/978-1-4939-0345-0_5 [doi]
AB  - Ex vivo-generated antigen-loaded dendritic cells (DC) have been shown to be 
      immunogenic in patients with cancer. Loading DC with autologous whole tumor 
      antigens is a strategy to arm DC against tumor without human leukocyte antigen 
      (HLA) restriction. Furthermore, this approach allows the presentation of a full 
      antigen range to the immune system. We describe the methods to obtain whole 
      antigens from autologous tumor tissues in order to load DC generated ex vivo from 
      patients with gastrointestinal cancer.
FAU - Rizzo, Manglio M
AU  - Rizzo MM
AD  - Gene Therapy Laboratory, Facultad de Ciencias Biomedicas, Universidad Austral, 
      Buenos Aires, Argentina.
FAU - Alaniz, Laura
AU  - Alaniz L
FAU - Mazzolini, Guillermo
AU  - Mazzolini G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Autoantigens)
SB  - IM
MH  - Antigens, Neoplasm/*immunology
MH  - Autoantigens/*immunology
MH  - Cell Culture Techniques
MH  - Dendritic Cells/cytology/*immunology
MH  - Gastrointestinal Neoplasms/immunology/therapy
MH  - Humans
MH  - Immunotherapy, Adoptive/*methods
EDAT- 2014/03/13 06:00
MHDA- 2014/11/15 06:00
CRDT- 2014/03/13 06:00
PHST- 2014/03/13 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/11/15 06:00 [medline]
AID - 10.1007/978-1-4939-0345-0_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1139:41-4. doi: 10.1007/978-1-4939-0345-0_5.