PMID- 24619674
OWN - NLM
STAT- MEDLINE
DCOM- 20141114
LR  - 20140312
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1139
DP  - 2014
TI  - Dendritic cells transfected with adenoviral vectors as vaccines.
PG  - 97-118
LID - 10.1007/978-1-4939-0345-0_10 [doi]
AB  - Dendritic cells (DCs) are critical to the initiation of a T-cell response. They 
      constitute the most potent antigen-presenting cell (APC) endowed with the unique 
      capacity to stimulate an antigen-specific T-cell responses by naive T cells. 
      Adenoviruses (Ad) have high transduction efficiency for many cell types including 
      cells of hematopoietic origin independent of their mitotic status, and 
      replication-defective Ad have demonstrated a safety profile clinically. Further, 
      Ad vectors provide a high level of transgene expression, and Ad-transduced DCs 
      can effectively present antigenic proteins. In this chapter, we outline a 
      functionally closed, good manufacturing protocol for the differentiation of 
      monocytes into DCs and transduction by Ad vectors. Basic functional and 
      phenotypic release assays are provided, as well as contrasting research 
      approaches for Ad-transduced DC-based vaccines.
FAU - Senesac, Joseph
AU  - Senesac J
AD  - Bellicum Pharmaceuticals, Houston, TX, USA.
FAU - Gabrilovich, Dmitry
AU  - Gabrilovich D
FAU - Pirruccello, Samuel
AU  - Pirruccello S
FAU - Talmadge, James E
AU  - Talmadge JE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cancer Vaccines)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Blood Component Removal
MH  - Cancer Vaccines/*genetics
MH  - Cell Adhesion
MH  - Cell Culture Techniques
MH  - Cell Separation
MH  - Cryopreservation
MH  - Dendritic Cells/cytology/*metabolism
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - Monocytes/cytology
MH  - Phenotype
MH  - Transduction, Genetic
MH  - Transfection/*methods
EDAT- 2014/03/13 06:00
MHDA- 2014/11/15 06:00
CRDT- 2014/03/13 06:00
PHST- 2014/03/13 06:00 [entrez]
PHST- 2014/03/13 06:00 [pubmed]
PHST- 2014/11/15 06:00 [medline]
AID - 10.1007/978-1-4939-0345-0_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1139:97-118. doi: 10.1007/978-1-4939-0345-0_10.