PMID- 24621048
OWN - NLM
STAT- MEDLINE
DCOM- 20160803
LR  - 20211021
IS  - 2045-7723 (Electronic)
IS  - 1079-0268 (Print)
IS  - 1079-0268 (Linking)
VI  - 38
IP  - 2
DP  - 2015 Mar
TI  - Curcumin modulates TLR4/NF-kappaB inflammatory signaling pathway following traumatic 
      spinal cord injury in rats.
PG  - 199-206
LID - 10.1179/2045772313Y.0000000179 [doi]
AB  - BACKGROUND: Curcumin, a polyphenolic compound extracted from the plant turmeric, 
      has protective effects on spinal cord injury (SCI) through attenuation of 
      inflammatory response. This study was designed to detect whether curcumin 
      modulates toll-like receptor 4 (TLR4) and the nuclear factor-kappa B (NF-kappaB) 
      inflammatory signaling pathway in the injured rat spinal cord following SCI. 
      METHODS: Adult male Sprague-Dawley rats were subjected to laminectomy at T8-T9 
      and compression with a vascular clip. There were three groups: (a) sham group; 
      (b) SCI group; and (g) SCI + curcumin group. We measured TLR4 gene and protein 
      expression by real-time polymerase chain reaction and western blot analysis; 
      NF-kappaB activity by electrophoretic mobility shift assay, inflammatory cytokines 
      tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 levels by 
      enzyme-linked immunosorbent assay, hindlimb locomotion function by Basso, 
      Beattie, and Bresnahan rating, spinal cord edema by wet/dry weight method, and 
      apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) analysis. RESULTS: The results showed that SCI induced the 
      up-regulation of TLR4, NF-kappaB, and inflammatory cytokines in the injured rat 
      spinal cord. Treatment with curcumin following SCI markedly down-regulated the 
      levels of these agents related to the TLR4/NF-kappaB inflammatory signaling pathway. 
      Administration of curcumin also significantly ameliorated SCI induced hind limb 
      locomotion deficits, spinal cord edema, and apoptosis. CONCLUSIONS: Post-SCI 
      curcumin administration attenuates the TLR4/NF-kappaB inflammatory signaling pathway 
      in the injured spinal cord, and this may be a mechanism whereby curcumin improves 
      the outcome following SCI.
FAU - Ni, Hongbin
AU  - Ni H
FAU - Jin, Wei
AU  - Jin W
FAU - Zhu, Tiansheng
AU  - Zhu T
FAU - Wang, Jing
AU  - Wang J
FAU - Yuan, Baoyu
AU  - Yuan B
FAU - Jiang, Jian
AU  - Jiang J
FAU - Liang, Weibang
AU  - Liang W
FAU - Ma, Zhengliang
AU  - Ma Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140103
PL  - England
TA  - J Spinal Cord Med
JT  - The journal of spinal cord medicine
JID - 9504452
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology/therapeutic use
MH  - Apoptosis
MH  - Curcumin/*pharmacology/therapeutic use
MH  - Locomotion
MH  - Male
MH  - NF-kappa B/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/*drug effects
MH  - Spinal Cord Injuries/drug therapy/*metabolism
MH  - Toll-Like Receptor 4/genetics/*metabolism
MH  - Up-Regulation
PMC - PMC4397202
OTO - NOTNLM
OT  - Curcumin
OT  - Inflammation
OT  - Nuclear factor-kappa B
OT  - Spinal cord injury
OT  - Toll-like receptor 4
EDAT- 2014/03/14 06:00
MHDA- 2016/08/04 06:00
PMCR- 2016/03/01
CRDT- 2014/03/14 06:00
PHST- 2014/03/14 06:00 [entrez]
PHST- 2014/03/14 06:00 [pubmed]
PHST- 2016/08/04 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - scm-38-199 [pii]
AID - 10.1179/2045772313Y.0000000179 [doi]
PST - ppublish
SO  - J Spinal Cord Med. 2015 Mar;38(2):199-206. doi: 10.1179/2045772313Y.0000000179. 
      Epub 2014 Jan 3.