PMID- 24622345
OWN - NLM
STAT- MEDLINE
DCOM- 20150529
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 3
DP  - 2014
TI  - Dendritic cells pulsed with leukemia cell-derived exosomes more efficiently 
      induce antileukemic immunities.
PG  - e91463
LID - 10.1371/journal.pone.0091463 [doi]
LID - e91463
AB  - Dendritic cells (DCs) and tumor cell-derived exosomes have been used to develop 
      antitumor vaccines. However, the biological properties and antileukemic effects 
      of leukemia cell-derived exosomes (LEXs) are not well described. In this study, 
      the biological properties and induction of antileukemic immunity of LEXs were 
      investigated using transmission electron microscopy, western blot analysis, 
      cytotoxicity assays, and animal studies. Similar to other tumor cells, leukemia 
      cells release exosomes. Exosomes derived from K562 leukemia cells (LEXK562) are 
      membrane-bound vesicles with diameters of approximately 50-100 mum and harbor 
      adhesion molecules (e.g., intercellular adhesion molecule-1) and immunologically 
      associated molecules (e.g., heat shock protein 70). In cytotoxicity assays and 
      animal studies, LEXs-pulsed DCs induced an antileukemic cytotoxic T-lymphocyte 
      immune response and antileukemic immunity more effectively than did LEXs and 
      non-pulsed DCs (P<0.05). Therefore, LEXs may harbor antigens and immunological 
      molecules associated with leukemia cells. As such, LEX-based vaccines may be a 
      promising strategy for prolonging disease-free survival in patients with leukemia 
      after chemotherapy or hematopoietic stem cell transplantation.
FAU - Yao, Ye
AU  - Yao Y
AD  - Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong 
      University School of Medicine, Shanghai, China.
FAU - Wang, Chun
AU  - Wang C
AD  - Department of Hematology, The First People's Hospital of Shanghai Affiliated to 
      Shanghai Jiaotong University, Shanghai, China.
FAU - Wei, Wei
AU  - Wei W
AD  - Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong 
      University School of Medicine, Shanghai, China.
FAU - Shen, Chang
AU  - Shen C
AD  - Department of Hematology, The First People's Hospital of Shanghai Affiliated to 
      Shanghai Jiaotong University, Shanghai, China.
FAU - Deng, Xiaohui
AU  - Deng X
AD  - Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong 
      University School of Medicine, Shanghai, China.
FAU - Chen, Linjun
AU  - Chen L
AD  - Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong 
      University School of Medicine, Shanghai, China.
FAU - Ma, Liyuan
AU  - Ma L
AD  - Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong 
      University School of Medicine, Shanghai, China.
FAU - Hao, Siguo
AU  - Hao S
AD  - Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong 
      University School of Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140312
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Dendritic Cells/*cytology/*immunology/ultrastructure
MH  - Exosomes/*pathology
MH  - Female
MH  - Humans
MH  - K562 Cells
MH  - Leukemia/*immunology/*pathology
MH  - Mice
MH  - Microscopy, Electron, Transmission
MH  - T-Lymphocytes, Cytotoxic/cytology/immunology
PMC - PMC3951359
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/03/14 06:00
MHDA- 2015/05/30 06:00
PMCR- 2014/03/12
CRDT- 2014/03/14 06:00
PHST- 2013/11/12 00:00 [received]
PHST- 2014/02/11 00:00 [accepted]
PHST- 2014/03/14 06:00 [entrez]
PHST- 2014/03/14 06:00 [pubmed]
PHST- 2015/05/30 06:00 [medline]
PHST- 2014/03/12 00:00 [pmc-release]
AID - PONE-D-13-47042 [pii]
AID - 10.1371/journal.pone.0091463 [doi]
PST - epublish
SO  - PLoS One. 2014 Mar 12;9(3):e91463. doi: 10.1371/journal.pone.0091463. eCollection 
      2014.