PMID- 24623193
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20190930
IS  - 1560-2281 (Electronic)
IS  - 1083-3668 (Linking)
VI  - 19
IP  - 7
DP  - 2014 Jul
TI  - Design and validation of a fiber optic point probe instrument for therapy 
      guidance and monitoring.
PG  - 71408
LID - 10.1117/1.JBO.19.7.071408 [doi]
AB  - Optical techniques for tissue diagnostics currently are experiencing tremendous 
      growth in biomedical applications, mainly due to their noninvasive, inexpensive, 
      and real-time functionality. Here, we demonstrate a hand-held fiber optic probe 
      instrument based on fluorescence/reflectance spectroscopy for precise tumor 
      delineation. It is mainly aimed for brain tumor resection guidance with clinical 
      adaptation to minimize the disruption of the standard surgical workflow and is 
      meant as a complement to the state-of-the-art fluorescence surgical microscopy 
      technique. Multiple light sources with fast pulse modulation and detection enable 
      precise quantification of protoporphyrin IX (PpIX), tissue optical properties, 
      and ambient light suppression. Laboratory measurements show the system is 
      insensitive to strong ambient light. Validation measurements of tissue phantoms 
      using nonlinear least squares support vector machines (LS-SVM) regression 
      analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 
      1000 nM, even in the presence of large variations in phantom optical properties. 
      The mean error is 3% for reduced scattering coefficient and 5% for blood 
      concentration. Diagnostic precision of 100% was obtained by LS-SVM classification 
      for in vivo skin tumors with topically applied 5-aminolevulinic acid during 
      photodynamic therapy. The probe could easily be generalized to other tissue types 
      and fluorophores for therapy guidance and monitoring.
FAU - Xie, Haiyan
AU  - Xie H
AD  - Lund University, Department of Physics, P.O. Box 118, SE-221 00 Lund, Sweden.
FAU - Xie, Zhiyuan
AU  - Xie Z
AD  - Lund University, Department of Physics, P.O. Box 118, SE-221 00 Lund, Sweden.
FAU - Mousavi, Monirehalsadat
AU  - Mousavi M
AD  - Lund University, Department of Physics, P.O. Box 118, SE-221 00 Lund, Sweden.
FAU - Bendsoe, Niels
AU  - Bendsoe N
AD  - Skane University Hospital, Department of Dermatology and Venereology, 
      Lasarettsgatan 15, SE-221 85 Lund, Sweden.
FAU - Brydegaard, Mikkel
AU  - Brydegaard M
AD  - Lund University, Department of Physics, P.O. Box 118, SE-221 00 Lund, Sweden.
FAU - Axelsson, Johan
AU  - Axelsson J
AD  - Lund University, Department of Physics, P.O. Box 118, SE-221 00 Lund, Sweden.
FAU - Andersson-Engels, Stefan
AU  - Andersson-Engels S
AD  - Lund University, Department of Physics, P.O. Box 118, SE-221 00 Lund, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
RN  - 0 (Protoporphyrins)
RN  - 88755TAZ87 (Aminolevulinic Acid)
RN  - C2K325S808 (protoporphyrin IX)
SB  - IM
MH  - Aminolevulinic Acid/chemistry
MH  - Animals
MH  - Calibration
MH  - Carcinoma, Squamous Cell/diagnosis/pathology
MH  - Equipment Design
MH  - Fiber Optic Technology/*instrumentation
MH  - Humans
MH  - Least-Squares Analysis
MH  - Light
MH  - Mice
MH  - Neoplasms/*diagnosis/pathology
MH  - *Optical Fibers
MH  - Phantoms, Imaging
MH  - Photochemotherapy/methods
MH  - Protoporphyrins/chemistry
MH  - Reproducibility of Results
MH  - Scattering, Radiation
MH  - Skin Neoplasms/diagnosis/pathology
MH  - Spectrophotometry/methods
MH  - Support Vector Machine
EDAT- 2014/03/14 06:00
MHDA- 2014/09/03 06:00
CRDT- 2014/03/14 06:00
PHST- 2013/11/20 00:00 [received]
PHST- 2014/02/11 00:00 [accepted]
PHST- 2014/03/14 06:00 [entrez]
PHST- 2014/03/14 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - 1849759 [pii]
AID - 10.1117/1.JBO.19.7.071408 [doi]
PST - ppublish
SO  - J Biomed Opt. 2014 Jul;19(7):71408. doi: 10.1117/1.JBO.19.7.071408.