PMID- 24624118
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140313
LR  - 20211022
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 5
DP  - 2014
TI  - Insulin receptor substrate-1 associates with small nucleolar RNA which 
      contributes to ribosome biogenesis.
PG  - 24
LID - 10.3389/fendo.2014.00024 [doi]
LID - 24
AB  - Insulin receptor substrates (IRSs) are well known to play crucial roles in 
      mediating intracellular signals of insulin-like growth factors (IGFs)/insulin. 
      Previously, we showed that IRS-1 forms high molecular mass complexes containing 
      RNAs. To identify RNAs in IRS-1 complexes, we performed ultraviolet (UV) 
      cross-linking and immunoprecipitation analysis using HEK293 cells expressing 
      FLAG-IRS-1 and FLAG-IRS-2. We detected the radioactive signals in the 
      immunoprecipitates of FLAG-IRS-1 proportional to the UV irradiation, but not in 
      the immunoprecipitates of FLAG-IRS-2, suggesting the direct contact of RNAs with 
      IRS-1. RNAs cross-linked to IRS-1 were then amplified by RT-PCR, followed by 
      sequence analysis. We isolated sequence tags attributed to 25 messenger RNAs and 
      8 non-coding RNAs, including small nucleolar RNAs (snoRNAs). We focused on the 
      interaction of IRS-1 with U96A snoRNA (U96A) and its host Rack1 (receptor for 
      activated C kinase 1) pre-mRNA. We confirmed the interaction of IRS-1 with U96A, 
      and with RACK1 pre-mRNA by immunoprecipitation with IRS-1 followed by Northern 
      blotting or RT-PCR analyses. Mature U96A in IRS-1(-/-) mouse embryonic 
      fibroblasts was quantitatively less than WT. We also found that a part of nuclear 
      IRS-1 is localized in the Cajal body, a nuclear subcompartment where snoRNA 
      mature. The unanticipated function of IRS-1 in snoRNA biogenesis highlights the 
      potential of RNA-associated IRS-1 complex to open a new line of investigation to 
      dissect the novel mechanisms regulating IGFs/insulin-mediated biological events.
FAU - Ozoe, Atsufumi
AU  - Ozoe A
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo 
      , Japan.
FAU - Sone, Meri
AU  - Sone M
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo 
      , Japan.
FAU - Fukushima, Toshiaki
AU  - Fukushima T
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo 
      , Japan ; Institute of Biomedical and Health Sciences, Hiroshima University , 
      Hiroshima , Japan.
FAU - Kataoka, Naoyuki
AU  - Kataoka N
AD  - Laboratory for Malignancy Control Research, Medical Innovation Center, Graduate 
      School of Medicine, Kyoto University , Kyoto , Japan.
FAU - Chida, Kazuhiro
AU  - Chida K
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo 
      , Japan.
FAU - Asano, Tomoichiro
AU  - Asano T
AD  - Institute of Biomedical and Health Sciences, Hiroshima University , Hiroshima , 
      Japan.
FAU - Hakuno, Fumihiko
AU  - Hakuno F
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo 
      , Japan.
FAU - Takahashi, Shin-Ichiro
AU  - Takahashi S
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo , Tokyo 
      , Japan.
LA  - eng
PT  - Journal Article
DEP - 20140304
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
PMC - PMC3941584
OTO - NOTNLM
OT  - RACK1
OT  - U96A
OT  - insulin receptor substrate
OT  - insulin-like growth factor
OT  - snoRNA
EDAT- 2014/03/14 06:00
MHDA- 2014/03/14 06:01
PMCR- 2014/01/01
CRDT- 2014/03/14 06:00
PHST- 2014/01/11 00:00 [received]
PHST- 2014/02/14 00:00 [accepted]
PHST- 2014/03/14 06:00 [entrez]
PHST- 2014/03/14 06:00 [pubmed]
PHST- 2014/03/14 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2014.00024 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2014 Mar 4;5:24. doi: 10.3389/fendo.2014.00024. 
      eCollection 2014.