PMID- 24624273
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140313
LR  - 20211022
IS  - 2005-6419 (Print)
IS  - 2005-7563 (Electronic)
IS  - 2005-6419 (Linking)
VI  - 66
IP  - 2
DP  - 2014 Feb
TI  - Role of calcium channels responsible for phenylephrine-induced contraction in rat 
      aorta 3 days after acute myocardial infarction.
PG  - 143-52
LID - 10.4097/kjae.2014.66.2.143 [doi]
AB  - BACKGROUND: Phenylephrine (PE) produces tonic contraction through involvement of 
      various calcium channels such as store-operated calcium channels (SOCCs) and 
      voltage-operated calcium channels (VOCCs). However, the relative contribution of 
      each calcium channel to PE-induced contraction has not been investigated in 
      isolated rat aorta of early acute myocardial infarction (AMI). METHODS: 
      Endothelium-denuded rat aortic rings from rats 3 days after AMI or sham-operated 
      (SHAM) rats were prepared in an organ chamber with Krebs-Ringer bicarbonate 
      solution for isometric tension recording. We assessed the PE dose-response 
      relationships in 2.5 mM calcium medium for both groups. The same procedure was 
      repeated using rings pretreated with the SOCC inhibitor 2-aminoethoxydiphenyl 
      borate, sarco/endoplasmic-reticulum calcium ATPase inhibitor thapsigargin (TG), 
      diacyl glycerol lipase inhibitor RHC80267, and sodium-calcium exchanger inhibitor 
      3,4-dichlorobenzamil hydrochloride for 30 minutes before addition of calcium. 
      When ongoing tonic contraction was sustained, dose-response curves to the VOCC 
      inhibitor nifedipine were obtained to assess the relative contribution of each 
      calcium channel under various conditions. RESULTS: The effect of SOCC induction 
      with TG pretreatment on PE-induced contraction was significantly lower in the AMI 
      group compared to the SHAM group. In addition, there were significant decreases 
      in the sensitivity and efficacy of the VOCC inhibitor nifedipine on PE-induced 
      contraction in the AMI group. CONCLUSIONS: Results suggest that the change of 
      vascular reactivity of PE in rat aorta 3 days after AMI is characterized by a 
      decreased contribution of L-type VOCCs. The enhanced VOCC-independent calcium 
      entry mechanisms after AMI can be mediated by enhanced capacitative calcium entry 
      through the activation of SOCCs.
FAU - Kim, Jung-Eun
AU  - Kim JE
AD  - Department of Anesthesiology and Pain Medicine, School of Medicine, Catholic 
      University of Daegu, Daegu, Korea.
FAU - Choi, Byung-Ki
AU  - Choi BK
AD  - Department of Anesthesiology and Pain Medicine, Chil-gok Catholic Hospital, 
      Catholic University of Daegu, Daegu, Korea.
FAU - Choi, Jun-Young
AU  - Choi JY
AD  - Department of Anesthesiology and Pain Medicine, School of Medicine, Catholic 
      University of Daegu, Daegu, Korea.
FAU - Ryu, Taeha
AU  - Ryu T
AD  - Department of Anesthesiology and Pain Medicine, School of Medicine, Catholic 
      University of Daegu, Daegu, Korea.
FAU - Roh, Woon Seok
AU  - Roh WS
AD  - Department of Anesthesiology and Pain Medicine, School of Medicine, Catholic 
      University of Daegu, Daegu, Korea.
FAU - Song, Seok-Young
AU  - Song SY
AD  - Department of Anesthesiology and Pain Medicine, School of Medicine, Catholic 
      University of Daegu, Daegu, Korea.
LA  - eng
PT  - Journal Article
DEP - 20140228
PL  - Korea (South)
TA  - Korean J Anesthesiol
JT  - Korean journal of anesthesiology
JID - 101502451
PMC - PMC3948442
OTO - NOTNLM
OT  - Calcium channels
OT  - Calcium influx
OT  - Myocardial infarction
OT  - Phenylephrine
EDAT- 2014/03/14 06:00
MHDA- 2014/03/14 06:01
PMCR- 2014/02/01
CRDT- 2014/03/14 06:00
PHST- 2013/11/12 00:00 [received]
PHST- 2014/01/13 00:00 [revised]
PHST- 2014/01/14 00:00 [accepted]
PHST- 2014/03/14 06:00 [entrez]
PHST- 2014/03/14 06:00 [pubmed]
PHST- 2014/03/14 06:01 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - 10.4097/kjae.2014.66.2.143 [doi]
PST - ppublish
SO  - Korean J Anesthesiol. 2014 Feb;66(2):143-52. doi: 10.4097/kjae.2014.66.2.143. 
      Epub 2014 Feb 28.