PMID- 24624874
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20181202
IS  - 0869-6047 (Print)
IS  - 0869-6047 (Linking)
IP  - 9
DP  - 2013
TI  - [Development of the quantitative immunochromatography tests for somatic deasease 
      markers detection].
PG  - 52-7
AB  - The purpose of work was creation of quantative immunochromatographic tests (ICT) 
      for measuring concentrations of the marker substance associated with somatic 
      diseases: immunoglobulin E (IgE), C-reactive protein (CRP) and fibrin D-dimer in 
      blood serum (plasma), which is carried out with the help of videodigital 
      analyzers of domestic development "Reflecom" and "Zondaj". ICT were designed in 
      sandwich-format, using colloid gold and monoclonal antibodies. It is shown, that 
      calibration curves received with the help of ICTand devices of videodigital 
      registration, are well approximated by exponential dependence Y = Aexp(-x/B)+y0, 
      where Y--device readings, x--analytes concentration, A, B, y0--constants. Samples 
      of blood serum (plasma), under investigation received in conditions of a hospital 
      from patients. Correlation with electrochemiluminescent immunoassay, enzyme 
      linked immunoassay, latex-agglutinations assay and immunochromatographic method 
      was observed. Sensitivity of the developed tests was 7,5 IU/ML for IgE, 5,7 ng/ml 
      for CRP, 500 ng/ml for fibrin D-dimer. The developed analytical complex- 
      videodigital analyzer "Zondaj" and ICT for quantitative measure of concentration 
      IgE, fibrin D-dimer and CRP- can be successfully applied in laboratory practice 
      and clinical laboratory researches alongside with actual immunochemistry methods.
FAU - Tret'iakov, S I
AU  - Tret'iakov SI
FAU - Basharova, L A
AU  - Basharova LA
FAU - Iarkov, S P
AU  - Iarkov SP
FAU - Titiov, A A
AU  - Titiov AA
FAU - Khramov, E N
AU  - Khramov EN
LA  - rus
PT  - Comparative Study
PT  - Journal Article
PL  - Russia (Federation)
TA  - Vestn Ross Akad Med Nauk
JT  - Vestnik Rossiiskoi akademii meditsinskikh nauk
JID - 9215641
RN  - 0 (Biomarkers)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Biomarkers/*analysis
MH  - C-Reactive Protein/analysis
MH  - Chromatography, Affinity/*methods
MH  - Humans
MH  - Immunoglobulin E/analysis
MH  - Reproducibility of Results
EDAT- 2013/01/01 00:00
MHDA- 2014/04/09 06:00
CRDT- 2014/03/15 06:00
PHST- 2014/03/15 06:00 [entrez]
PHST- 2013/01/01 00:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
PST - ppublish
SO  - Vestn Ross Akad Med Nauk. 2013;(9):52-7.