PMID- 24625230
OWN - NLM
STAT- MEDLINE
DCOM- 20140916
LR  - 20211021
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 14
DP  - 2014 Mar 13
TI  - Inhibition of HSP70 reduces porcine reproductive and respiratory syndrome virus 
      replication in vitro.
PG  - 64
LID - 10.1186/1471-2180-14-64 [doi]
AB  - BACKGROUND: Successful viral infection requires the involvement of host cellular 
      factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by 
      numerous viruses to promote the folding, maturation, or assembly of viral 
      proteins. We have previously shown that HSP70 is significantly elevated in 
      porcine reproductive and respiratory syndrome virus (PRRSV)-infected lungs, 
      suggesting HSP70 may play a potential role during PRRSV infection. In this study, 
      we tried to investigate the role of HSP70 during PRRSV infection. RESULTS: In 
      this study, we observed that PRRSV infection induced the expression of HSP70. The 
      down-regulation of HSP70 using quercetin, a HSPs synthesis inhibitor, or small 
      interfering RNAs (siRNA) reduced the viral protein level and viral production. 
      Notably, these inhibitory effects on PRRSV infection could be attenuated by heat 
      shock treatment. In addition, HSP70 was found to colocalize with the viral 
      double-stranded RNA (dsRNA) and knockdown of HSP70 decreased the dsRNA levels, 
      suggesting HSP70 is involved in the formation of viral replication and 
      transcription complex (RTC) and thus affects the viral replication. CONCLUSIONS: 
      Our study revealed that HSP70 is an essential host factor required for the 
      replication of PRRSV. The inhibition of HSP70 significantly reduced PRRSV 
      replication, which may be applied as an effective antiviral strategy.
FAU - Gao, Jintao
AU  - Gao J
FAU - Xiao, Shuqi
AU  - Xiao S
FAU - Liu, Xiaohong
AU  - Liu X
FAU - Wang, Liangliang
AU  - Wang L
FAU - Ji, Qianqian
AU  - Ji Q
FAU - Mo, Delin
AU  - Mo D
FAU - Chen, Yaosheng
AU  - Chen Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, P, R, China. chyaosh@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140313
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Gene Knockdown Techniques
MH  - HSP70 Heat-Shock Proteins/antagonists & inhibitors/*metabolism
MH  - *Host-Pathogen Interactions
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Quercetin/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Swine
MH  - *Virus Replication
PMC - PMC3984673
EDAT- 2014/03/15 06:00
MHDA- 2014/09/17 06:00
PMCR- 2014/03/13
CRDT- 2014/03/15 06:00
PHST- 2014/01/06 00:00 [received]
PHST- 2014/03/07 00:00 [accepted]
PHST- 2014/03/15 06:00 [entrez]
PHST- 2014/03/15 06:00 [pubmed]
PHST- 2014/09/17 06:00 [medline]
PHST- 2014/03/13 00:00 [pmc-release]
AID - 1471-2180-14-64 [pii]
AID - 10.1186/1471-2180-14-64 [doi]
PST - epublish
SO  - BMC Microbiol. 2014 Mar 13;14:64. doi: 10.1186/1471-2180-14-64.